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Abstract

An alkaliphilic, moderately halophilic, heterotrophic, rod-shaped, spore-forming bacterium (M30) was isolated from a sediment sample collected from a soda lake (Lake Magadi, Tanzania). Strain M30 was strictly aerobic, catalase-positive, oxidase-negative and non-motile. Growth occurred at 12–43 °C (optimum, 25–30 °C), at pH 8.0–12 (optimum, pH 9.5–10) and at salinities of 0.5–15 % (w/v) NaCl (optimum 5 %). It utilized various sugars and organic acids as sole carbon sources and was positive for amylase, cellulase, gelatinase, protease and xylanase activities. The cell-wall peptidoglycan contained -diaminopimelic acid and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content was 48.9 mol%. The predominant menaquinone was MK-7 and the major fatty acids (>10 %) comprised anteiso-C, iso-C, and anteiso-C. Phylogenetic analysis based on 16S rRNA gene sequence affiliated M30 to the genus and showed the highest similarities to FJAT-45347 (96.4 %) and K1-5 (96.2 %). Based on the data from the current polyphasic study, M30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M30 (=JCM 32118=CGMCC 1.16739=MCC 3010).

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2019-10-15
2020-01-24
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References

  1. Logan NA, De Vos P. Bacillus Bergey's Manual of Systematics of Archaea and Bacteria NJ: John Wiley & Sons, Inc., in association with Bergey's Manual Trust; 2015; pp 1– 163
    [Google Scholar]
  2. Rees HC, Grant WD, Jones BE, Heaphy S. Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods. Extremophiles 2004;8: 63– 71 [CrossRef]
    [Google Scholar]
  3. Grant WD, Sorokin DY. Distribution and diversity of soda lake alkaliphiles In Horikoshi K. editor Extremophiles Handbook Tokyo: Springer; 2011; pp 27– 47
    [Google Scholar]
  4. Machin EV, Asem MD, Salam N, Iriarte A, Langleib M et al. Nesterenkonia natronophila sp. nov., an alkaliphilic actinobacterium isolated from a soda lake, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 2019;69: 1960– 1966 [CrossRef]
    [Google Scholar]
  5. Barrow GI, Feltham RKA. Cowan and Steel's Manual for Identification of Medical Bacteria Cambridge: Cambridge University Press; 2003
    [Google Scholar]
  6. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM; 2007; pp 330– 393
    [Google Scholar]
  7. Menes RJ, Viera CE, Farías ME, Seufferheld MJ. Halopeptonella vilamensis gen. nov. sp. nov., a halophilic strictly aerobic bacterium of the family Ectothiorhodospiraceae. Extremophiles 2016;20: 19– 25 [CrossRef]
    [Google Scholar]
  8. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996;42: 989– 1005 [CrossRef]
    [Google Scholar]
  9. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66: 199– 202 [CrossRef]
    [Google Scholar]
  10. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990;13: 128– 130 [CrossRef]
    [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67: 1613– 1618 [CrossRef]
    [Google Scholar]
  12. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4: 406– 425 [CrossRef]
    [Google Scholar]
  13. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17: 368– 376 [CrossRef]
    [Google Scholar]
  14. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20: 406– 416 [CrossRef]
    [Google Scholar]
  15. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33: 1870– 1874 [CrossRef]
    [Google Scholar]
  16. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16: 111– 120 [CrossRef]
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39: 783– 791 [CrossRef]
    [Google Scholar]
  18. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19: 455– 477 [CrossRef]
    [Google Scholar]
  19. Zerbino DR, Birney E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 2008;18: 821– 829 [CrossRef]
    [Google Scholar]
  20. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013;29: 1072– 1075 [CrossRef]
    [Google Scholar]
  21. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST server: rapid annotations using subsystems technology. BMC Genomics 2008;9: 75 [CrossRef]
    [Google Scholar]
  22. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110: 1281– 1286 [CrossRef]
    [Google Scholar]
  23. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. Dna-Dna hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007;57: 81– 91 [CrossRef]
    [Google Scholar]
  24. Borsodi AK, Márialigeti K, Szabó G, Palatinszky M, Pollák B et al. Bacillus aurantiacus sp. nov., an alkaliphilic and moderately halophilic bacterium isolated from hungarian soda lakes. Int J Syst Evol Microbiol 2008;58: 845– 851 [CrossRef]
    [Google Scholar]
  25. Liu B, Liu GH, Wang XY, Wang JP, Zhu YJ et al. Bacillus populi sp. nov. isolated from Populus euphratica rhizosphere soil of the taklamakan desert. Int J Syst Evol Microbiol 2018;68: 155– 159 [CrossRef]
    [Google Scholar]
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