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Volume 70, Issue 1

sp. nov., an alkaliphilic bacterium isolated from a soda lake Free

Abstract

An alkaliphilic, moderately halophilic, heterotrophic, rod-shaped, spore-forming bacterium (M30) was isolated from a sediment sample collected from a soda lake (Lake Magadi, Tanzania). Strain M30 was strictly aerobic, catalase-positive, oxidase-negative and non-motile. Growth occurred at 12–43 °C (optimum, 25–30 °C), at pH 8.0–12 (optimum, pH 9.5–10) and at salinities of 0.5–15 % (w/v) NaCl (optimum 5 %). It utilized various sugars and organic acids as sole carbon sources and was positive for amylase, cellulase, gelatinase, protease and xylanase activities. The cell-wall peptidoglycan contained -diaminopimelic acid and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content was 48.9 mol%. The predominant menaquinone was MK-7 and the major fatty acids (>10 %) comprised anteiso-C, iso-C, and anteiso-C. Phylogenetic analysis based on 16S rRNA gene sequence affiliated M30 to the genus and showed the highest similarities to FJAT-45347 (96.4 %) and K1-5 (96.2 %). Based on the data from the current polyphasic study, M30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M30 (=JCM 32118=CGMCC 1.16739=MCC 3010).

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Keyword(s): alkaliphile , Bacillus natronophilus sp. nov , Bacillus sp , moderately halophilic and soda lake
© 2020 The Authors
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2020-02-03
2024-04-20
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Bacillus natronophilus sp. nov., an alkaliphilic bacterium isolated from a soda lake
Int J Syst Evol Microbiol 70, 562 (2020); https://doi.org/10.1099/ijsem.0.003792
/content/journal/ijsem/10.1099/ijsem.0.003792
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