%0 Journal Article %A Zhao, Zhuo-li %A Ming, Hong %A Ding, Chen-Long %A Ji, Wei-Li %A Cheng, Li-Jiao %A Niu, Ming-ming %A Zhang, Yan-min %A Zhang, Ling-Yu %A Meng, Xiao-Lin %A Nie, Guo-Xing %T Azospirillum thermophilum sp. nov., isolated from a hot spring %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 1 %P 550-554 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003788 %K polyphasic taxonomy %K hot spring %K Azospirillum thermophilum sp. nov. %I Microbiology Society, %X A novel Gram-staining negative, aerobic, motile by flagellum, rod-shaped bacterium, designated CFH 70021T was isolated from a hot spring soil sample collected from Tengchong, Yunnan province, PR China. Growth of CFH 70021T occurred at 15–50 °C (optimum 50 °C), pH 5.0–7.0 (optimum pH 7.0) and with 0–3.0 % (w/v) NaCl (optimum 0 %, w/v). The genome of CFH 70021T consisted of four complete circular chromosomes and five plasmids, the genomic DNA G+C content was 69.3 mol%. Comparison of the 16S rRNA gene sequences indicated that CFH 70021T represented a member of the genus Azospirillum and showed close relationship with the type strains of Azospirillum agricola CC-HIH038T (97.8 %), Azospirillum rugosum IMMIB AFH-6T (97.6 %), Azospirillum doebereinerae GSF71T (97.6 %), Azospirillum thiophilum DSM 21654T (97.4 %) and Azospirillum picis IMMIB TAR-3T (97.2 %). The polar lipids of CFH 70021T contained diphosphatidylglycerol, phosphatidylmehtylethanolamine, phosphatidylglycerol, phosphatidylcholine, two aminolipids and an unidentified phospholipid. The predominant cellular fatty acids (>10 %) included C19:0cyclo ω8c (11.4 %), C16 : 0 (27.6 %) and summed feature 8 (C18:1ω7c/C18:1ω6c, 40.9 %). The major isoprenoid quinone was Q-10. On the basis of the low ANIb result (<78 %) and different phenotypic and chemotaxonomic characters, we conclude that strain CFH 70021T represents a novel member of the genus Azospirillum , for which the name Azospirillum thermophilum sp. nov. is proposed. The type strain is CFH 70021T (=KCTC 62259T= CCTCC AB2018121T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003788