RT Journal Article SR Electronic(1) A1 Choi, Gyu-min A1 Lee, Soon Youl A1 Kim, Sang Yong A1 Wee, Ji-Hyang A1 Im, Wan-TaekYR 2020 T1 Hydrogenophaga borbori sp. nov., isolated from activated sludge JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 1 SP 555 OP 561 DO https://doi.org/10.1099/ijsem.0.003787 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-negative, yellowish-pigmented bacterial strain, designated LA-38T, was isolated from activated sludge of wastewater treatment plants in Hanam city, South Korea. Cell of LA-38T were rod-shaped, aerobic, motile and non-spore-forming. In phylogenetic analyses based on 16S rRNA genes, LA-38T clustered with species of the genus Hydrogenophaga and appeared closely related to Hydrogenophaga intermedia DSM 5680T (99.2 % similarity), Hydrogenophaga palleronii DSM 63T (98.2 %), Hydrogrenophaga laconesensis KCTC 42478T (98.1 %), Hydrogenophaga. atypica DSM 15342T (98.1 %), Hydrogenophaga defluvii DSM 15341T (98.0 %) and Hydrogenophaga taeniospiralis DSM 2082T (97.2 %). The average nucleotide identities between LA-38T and the closely related strains were 79.3–88.5 %, indicating that LA-38T represents a novel species of the genus Hydrogenophaga . The DNA G+C content of the genomic DNA was 69.9 mol% and ubiquinone Q-8 was the predominant respiratory quinone. The major cellular fatty acids (>5 %) were C16 : 0, cyclo-C19 : 0, C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3), and C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8). The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine, the major polyamines were 2-hydroxyputrescine and putrescine. ANI calculation, physiological and biochemical characteristics indicated that LA-38T represents a novel species of the genus Hydrogenophaga , for which the name Hydrogenophaga borbori sp. nov. is proposed. The type strain is LA-38T (=KACC 19730T=LMG 30805T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003787