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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 1

sp. nov., a novel actinomycete isolated from the cow dung Free

Abstract

A novel actinobacterial strain, designated NEAU-LL90, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east PR China and characterized by using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those members of the genus . The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinone detected was MK-8(H4, ω-cycl). Major fatty acids (>10 %) were identified as C, Cω9, C and 10-methyl C. Mycolic acids were present. The results of 16S rRNA gene sequence analysis showed that strain NEAU-LL90 belongs to the genus with high sequence similarity to JCM11894 (98.1 %), similarities to other type strains of species of the genus were found to be lower than 98.0 %. Furthermore, a combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-LL90 could be distinguished from its closest relative. Therefore, it is proposed that strain NEAU-LL90 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-LL90 (=CGMCC 4.7500=JCM 32663).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA genex , Nocardia stercoris sp. nov. and polyphasic analysis
Funding
This study was supported by the:
  • the National Natural Youth Science Foundation of China (Award 31701858)
    • Principle Award Recipient: Junwei Zhao
© 2020 The Authors
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2020-02-03
2025-04-19
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Nocardia stercoris sp. nov., a novel actinomycete isolated from the cow dung
Int J Syst Evol Microbiol 70, 493 (2020); https://doi.org/10.1099/ijsem.0.003784
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