1887

Abstract

A yellow-coloured, Gram-negative, motile, strictly aerobic bacterial strain, designated strain DAC4, was isolated from a soil sample collected at Ahnmok Beach (Busan, Republic of Korea). The cells of strain DAC4 were rod-shaped and the colonies that formed were round and convex. The results of phylogenetic analysis based on the 16S rRNA gene sequence of strain DAC4 revealed that the bacterium belongs to the genus , family , and that it was most closely related to DSM 18422 (98.01 %), KACC 19189 (97.76 %), KCTC 62179(97.30 %), KCTC 12629 (97.16 %) and KCTC 22476 (97.05 %). The major respiratory quinone was Q-10, and the major cellular fatty acids were summed feature 8 (C ω7) and summed feature 3 (C ω7/C ω6). The whole genome DNA G+C content of strain DAC4 was 62.16 mol%. Phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipids, phosphatidylglycerol, phosphatidylcholine, four undefined glycolipids and an undefined lipid were detected in strain DAC4, and the strain had sym-homospermidine as a major polyamine. The DNA–DNA hybridization and average nucleotide identity values between strain DAC4 and the closely related taxa and were 75.5/23.5 % and 73.5 /18.5%, respectively. The fluorimetric DNA–DNA hybridization results showed that strain DAC4 and , and have 37.1, 35.2 and 32.2 % DNA similarity, respectively. Based on phylogenetic, phenotypic and chemotaxonomic distinctiveness, strain DAC4 (=KCTC 62107=JCM 32377) is classified as a novel species of the genus , for which the name sp. nov. is proposed.

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2019-10-09
2019-10-22
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