1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 1

sp. nov., sp. nov. and subsp. subsp. nov., isolated from human faeces, and creation of subsp. subsp. nov. Free

Abstract

Three groups of Gram-stain-negative, obligately anaerobic, rod or coccoid-shaped bacteria, which were phylogenetically assigned in the genus belonging to the family in the phylum , were isolated from the faecal samples of healthy Japanese humans. Group I (strains 5CBH24 and 6CPBBH3) showed highest 16S rRNA gene sequence similarity to ‘’ ph8 (99.73 %). Group II (strain 5CPEGH6) was related to WAL 8301 (96.82 %). Ten strains of group III (3BBH6, 5CPYCFAH4, 5NYCFAH2 and others) were related to DSM 19147 (98.96 %). Group I could be differentiated from other strains by the ability to hydrolyse aesculin and the lack of catalase activity. Strain 5CPEGH6 could be differentiated from JCM 16773 by the inability to hydrolyse aesculin and the lack of catalase activity, and so on. Phenotypic characteristics of group III were similar to those of JCM 16771. Strains 5CBH24, 6CPBBH3 and ‘’ ph8 shared 98.8–98.9 % average nucleotide identity (ANI) with each other. In addition, the DNA–DNA hybridization (DDH) values among three strains were 86.7–89.4 %. Strain 5CPEGH6 showed relatively low values (≤ 84.4 % for ANI ; ≤26.2 % for DDH) with other strains. Three strains in the group III (3BBH6, 5CPYCFAH4 and 5NYCFAH2) shared 97.9–99.9% ANI with each other. These three strains showed 96.9–97.3 % ANI with DSM 19147. The DDH values of strains 3BBH6, 5CPYCFAH4 and 5NYCFAH2 among themselves were 80.5–99.8 %, while those compared to DSM 19147 were 71.0–73.4 %. On the basis of the collected data, three novel species, sp. nov. (5CBH24=JCM 32850=DSM 108979), sp. nov. (5CPEGH6=JCM 32848=DSM 108978) and subsp. subsp. nov. (3BBH6=JCM 32839=DSM 108977), are proposed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Alistipes , hsp60 , human faeces and quinone
Funding
This study was supported by the:
  • RIKEN
    • Principle Award Recipient: Moriya Ohkuma
  • Japan Agency for Medical Research and Development (Award JP18gm6010007)
    • Principle Award Recipient: Mitsuo Sakamoto
© 2020 The Authors
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2020-02-03
2024-10-04
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Alistipes communis sp. nov., Alistipes dispar sp. nov. and Alistipes onderdonkii subsp. vulgaris subsp. nov., isolated from human faeces, and creation of Alistipes onderdonkii subsp. onderdonkii subsp. nov.
Int J Syst Evol Microbiol 70, 473 (2020); https://doi.org/10.1099/ijsem.0.003778
/content/journal/ijsem/10.1099/ijsem.0.003778
/content/journal/ijsem/10.1099/ijsem.0.003778
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