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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 1

sp. nov., a novel endophytic bacterium isolated from root of (Pilger) Rehd Free

Abstract

A Gram-staining-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M5HDSG1-1, was originally isolated from a surface-sterilized root of (Pilger) Rehd. in Guizhou, PR China. This bacterium was tested by a polyphasic approach to determine its taxonomic position. A 16S rRNA gene-based phylogenetic analysis revealed that M5HDSG1-1 had the greatest similarity to the type strain of DSM 15077 (99.1 %). The average nucleotide identity values between M5HDSG1-1 and DSM 15077 and NBRC 13626 were 73.3 and 72.8 %, respectively. The digital DNA–DNA hybridization values between M5HDSG1-1 and DSM 15077 and NBRC 13626 were 20.1 and 20.6 %, respectively, which were below the recommended thresholds. M5HDSG1-1 grew at a pH range of 6.0–12.0 (optimum, 7.0–8.0), at temperatures between 10 and 45 °C (optimum, 30 °C) and at 0–2 % (w/v) NaCl (optimum, 1 %). Neither substrate nor aerial mycelia was formed, and no diffusible pigments were observed on the media tested. The predominant isoprenoid quinone was menaquinone-7 (MK-7). The major fatty acids were -C, and -C. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified phospholipid. The DNA G+C content was 37.5 mol%. According to the phylogeneic, phenotypic and chemotaxonomic data, M5HDSG1-1 was clearly distinguishable from other species with validly published names in the genus and should therefore be classified as representing a novel species, and we suggest the name sp. nov. The type strain is M5HDSG1-1 (=JCM 33117=CGMCC 1.13668).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus , M5HDSG1-1 , novel species and taxonomic position
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 81960642)
    • Principle Award Recipient: Li Tuo
  • the Bidding Project of Zunyi Medical University (Award F-614)
    • Principle Award Recipient: Yun Liu
  • the PhD Start-up Fund of Zunyi Medical University (Award F-800)
    • Principle Award Recipient: Yun Liu
  • Youth Science and technology personnel growth project of Guizhou Provincial Education Department (Award Qian Jiao He KY Zi[2016]200))
    • Principle Award Recipient: Li Tuo
  • Science and Technology Foundation of Guizhou Province (Award Qian Ke He Jichu[2019]1347)
    • Principle Award Recipient: Li Tuo
  • National Natural Science Foundation of China (Award 81603079)
    • Principle Award Recipient: Li Tuo
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.003776
2019-10-15
2025-01-23
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Bacillus taxi sp. nov., a novel endophytic bacterium isolated from root of Taxus chinensis (Pilger) Rehd
Int J Syst Evol Microbiol 70, 481 (2020); https://doi.org/10.1099/ijsem.0.003776
/content/journal/ijsem/10.1099/ijsem.0.003776
/content/journal/ijsem/10.1099/ijsem.0.003776
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