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Volume 70, Issue 1

gen. nov., sp. nov., isolated from marine mangrove sediment, and reclassification of as comb. nov. Free

Abstract

Strain GS-14 was isolated from a mangrove sediment sample collected at Beilun Estuary National Nature Reserve, Guangxi Province, PR China. Cells were Gram-stain-negative, strictly aerobic and rod-shaped with a polar flagellum. Optimal growth occurred in the presence of 3–6 % (w/v) NaCl, at pH 6–8 and at a temperature of 37 °C. The predominant polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone 8 (Q-8) was the sole respiratory quinone. The major fatty acids (>10 % of the total fatty acids) were summed feature 3 (Cω7 and/or Cω6) and C. The DNA G+C content was 47.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GS-14 had the highest sequence similarity to WH169 (96.63 %), AK49 (96.56 %) and JW12 (96.22 %). In addition, the OrthoANIu value and dDDH values calculated from the genomes of strain GS-14 and WH169 were 79.5 and 21.9 %, respectively. Based on the polyphasic taxonomic results, strain GS-14 is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is GS-14 (=KCTC 72401=MCCC 1K03622). Because WH169 clustered with strain GS-14 in the phylogenetic trees and was clearly separated from the two species within the genus , it is reclassified as a member of the genus as comb. nov. (type strain WH169=CGMCC 1.8995=LMG 25283). The type species of the genus is gen. nov., comb. nov.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): mangrove wetland sediment , Marisediminitalea mangrovi , polyphasic taxonomy and reclassification
Funding
This study was supported by the:
  • Xiamen University (Award 201910384171)
    • Principle Award Recipient: Shiyin Wu
  • Natural Science Foundation of Fujian Province (Award 2019J01023)
    • Principle Award Recipient: Hong Xu
  • National Natural Science Foundation of China (Award 41676101 and 41476095)
    • Principle Award Recipient: Hong Xu
© 2020 The Authors
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2020-02-03
2024-04-25
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Marisediminitalea mangrovi gen. nov., sp. nov., isolated from marine mangrove sediment, and reclassification of Aestuariibacter aggregatus as Marisediminitalea aggregata comb. nov.
Int J Syst Evol Microbiol 70, 457 (2020); https://doi.org/10.1099/ijsem.0.003773
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