sp. nov., a rhizobium isolated from a root nodule in Norway Free

Abstract

Strain 10.2.2 was isolated from a root nodule of a plant growing near Skammestein (Norway). Phenotypic and chemotaxonomic characterization revealed that colonies grown on yeast–mannitol broth agar were circular, convex and slimy. Growth occurred at 28 °C in 0–1 % NaCl and in a pH range from above 4 to 10. Cells were resistant to kanamycin and phosphomycin. They could assimilate carbon sources such as -lysine, -mannose, -mannitol, and -alanine. Major fatty acids found in the organism were 11-methyl C ω7, C , C ω7, C and C cyclo ω8. Genome sequencing and characterization of the genome revealed its size to be 8.27 Mbp with a G+C content of 62.4 mol%. Phylogenetic analyses based on the 16S rRNA gene and housekeeping gene alignments placed this strain within the genus . Pairwise genome-wide average nucleotide identity values supported that strain 10.2.2 represents a new species, for which we propose the name sp. nov. with the type strain 10.2.2 (=DSM 108834=LMG 31153).

Funding
This study was supported by the:
  • Deutscher Akademischer Austauschdienst (Award 57214223)
    • Principle Award Recipient: Tolganay Kabdullayeva
  • Deutsche Forschungsgemeinschaft, http://dx.doi.org/10.13039/501100001659 (Award MA7269–1/1)
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2020-02-03
2024-03-28
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