@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003768, author = "Li, Qinyuan and Rückert, Christian and Li, Guiding and Huang, Ping and Schneider, Olha and Kalinowski, Jörn and Jiang, Yi and Zotchev, Sergey B. and Jiang, Chenglin", title = "Prauserella flavalba sp. nov., a novel species of the genus Prauserella, isolated from alkaline soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "1", pages = "380-387", doi = "https://doi.org/10.1099/ijsem.0.003768", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003768", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Prauserella flavalba sp. nov.", keywords = "16S rRNA gene", keywords = "secondary metabolite biosynthesis gene clusters", keywords = "polyhasic taxonomy", abstract = "A Gram-strain positive, mycelium-forming actinomycete, YIM 121212T, was isolated from an alkaline soil sample collected in Yunnan province, PR China. Classification using a polyphasic approach indicated that YIM 121212T represents a member of the genus Prauserella , and is closely related to Prauserella coralliicola SCSIO 11529T (99.31 %), Prauserella endophytica SP28S-3T (99.17 %), Prauserella soli 12-833T (97.43 %), Prauserella oleivorans RIPIT (97.03 %), Prauserella marina MS498T (96.74 %), Prauserella rugosa DSM 43194T (96.54 %) and Prauserella muralis 05-Be-005T (95.92 %). Average nucleotide identity values (ANI) of YIM 121212T to P. coralliicola DSM 45821T and P. endophytica CGMCC 4.7182T were 93.1 and 92.8 %, respectively, which were lower than the threshold of 95 %. The digital DNA–DNA hybridization (dDDH) values between YIM 121212T and these two species were 50.8 and 49.9 %, respectively and thus were also well below the cut off value (>70 %) for species delineation. The DNA G+C content of YIM 121212T is 70.8 mol%. Major fatty acids are iso-C16 : 0, iso-C16 : 1H, C16 : 1ω7c/iso-C15 : 0 2OH, C17 : 1ω6c, and C17 : 1ω8c. The predominant menaquinone is MK-9(H4). The polar lipid profile consists of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmethylethanolamine (PME), phosphatidylinositol (PI), and phosphatidylinositol mannoside (PIM). The draft genomes were further analyzed for the presence of secondary metabolite biosynthesis (SMB) gene clusters. On the basis of the above observations, YIM121212T can be distinguished from closely related species belonging to the genus Prauserella . Thus, YIM121212T represents a novel species of the genus Prauserella , for which the name Prauserella flavalba sp. nov. is proposed. The type strain is YIM121212T (=CCTCC AA 2013011T=DSM 45973T).", }