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Volume 70, Issue 2

Gryllotalpicola protaetiae sp. nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli Free

Abstract

A bacterial strain, designated 2DFW10M-5, was isolated from gut of the larva of collected in the Republic of Korea. Cells of the strain were Gram-stain-positive, non-motile and rod-shaped. The strain grew at the range of 10–37 °C (optimum, 28–30 °C) and pH 4.0–8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium. It was catalase-positive and oxidase-negative. The 16S rRNA gene sequence of strain 2DFW10M-5 showed the highest similarity to RU-04 (98.4 %), KIS12-7 (98.2 %), PU-02 (97.5 %), RU-16 (97.4 %) and TS-56 (97.2 %). The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5 fell into the radius of the genus . The predominant fatty acid was ω-cyclohexyl-C. The polar lipids of strain 2DFW10M-5 were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids. The detected isoprenoid quinones were MK-11 (61.0 %), MK-10 (33.7 %) and MK-12 (5.3 %). The peptidoglycan contained - and -alanine, -glutamic acid, glycine, -serine and -lysine with -lysine as the diamino acid. The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5 was 69.2 mol%. On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2DFW10M-5 (=KACC 19316=NBRC 113049).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Gryllotalpicola protaetiae , new taxa , Protaetia brevitarsis seulensis and taxonomy
Funding
This study was supported by the:
  • National Institute of Agricultural Sciences (Award PJ013549)
    • Principle Award Recipient: Soon-Wo Kwon
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.003767
2019-11-01
2024-04-16
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Gryllotalpicola protaetiae sp. nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli
Int J Syst Evol Microbiol 70, 715 (2020); https://doi.org/10.1099/ijsem.0.003767
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