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Volume 70, Issue 1

gen. nov., sp. nov., a piezotolerant bacterium isolated from a deep-sea sediment sample of the New Britain Trench Open Access

Abstract

A novel alphaproteobacterium, strain WS11, was isolated from a deep-sea sediment sample collected from the New Britain Trench. The full-length 16S rRNA gene of strain WS11 had the highest sequence similarity of 97.6 % to JC85, followed by DSM 7292 (96.9 %) and Po 20/26 (96.8 %). Phylogenetic analysis of concatenated 16S rRNA, and gene sequences showed that strain WS11 was deeply separated from the species within the family . Phylogenomic analysis based on the whole-genome protein sequences showed that strain WS11 formed an independent monophyletic branch in the family , paralleled with the species in the families and within the order es. Cells were Gram-stain-negative, oxidase- and catalase-positive, and aerobic short rods (1.5–2.4×0.9–1.0 µm). Growth was observed at salinities ranging from 0 to 5% (optimum, 1 %), from pH 6.5 to 9 (optimum, pH 7) and at temperatures between 20 and 30 °C (optimum, 28 °C). Strain WS11 was piezotolerant, growing optimally at 0.1 MPa (range 0.1–70 MPa). The main fatty acid was summed feature 8 (C 7/C ). The sole respiratory quinone was ubiquinone-10 (Q-10). The predominant polar lipids were phosphatidylcholine, two unidentified aminophospholipids and an unidentified phospholipid. The genome size was about 4.36 Mbp and the G+C content was 62.3 mol%. The combined genotypic and phenotypic data show that strain WS11 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed (type strain WS11=MCCC 1K03498=KCTC 62439).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Georhizobium profundi , piezotolerant and Rhizobiaceae
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41706146)
    • Principle Award Recipient: Junwei Cao
  • National Key R&D Program of China (Award 2018YFC0310600)
    • Principle Award Recipient: Jiasong Fang
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2020-02-03
2024-04-19
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Georhizobium profundi gen. nov., sp. nov., a piezotolerant bacterium isolated from a deep-sea sediment sample of the New Britain Trench
Int J Syst Evol Microbiol 70, 373 (2020); https://doi.org/10.1099/ijsem.0.003766
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