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Abstract

A novel Gram-stain-negative, rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated A6024, was isolated from coastal seawater near Rizhao, PR China (119.61° E 35.47° N). The organism grew optimally at 28 °C, in pH 6.0–7.0 and in the presence of 3.0 % (w/v) NaCl. The strain required seawater or artificial seawater for growth and NaCl alone did not support growth. Strain A6024 contained ubiquinone 10 as the sole respiratory quinone and C 7 (75.2 %) as the most abundant fatty acid. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain A6024 was 59.9 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to MA1-10, GYSW-22 and CV919-312 with 98.3, 97.6 and 97.4 % sequence similarities, respectively. The calculated average nucleotide identity values and DNA–DNA hybridization values between strain A6024 and the phylogenetically related species were in the range 76.0–85.6 % and 19.6–29.4 %, respectively. On the basis of the results of polyphasic analyses, strain A6024 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A6024 (=KCTC 72114=MCCC 1K03595).

Funding
This study was supported by the:
  • the Henan Province University Youth Researcher Support Project (Award 2016GGJS-102)
    • Principle Award Recipient: Yan Wang
  • the Foundation of He'nan Science and Technology Agency of China
    • Principle Award Recipient: Yan Wang
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2020-02-03
2021-10-28
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