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Abstract

Strain TLA-22, isolated from a cold spring in Taiwan, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile by means of a single polar flagellum, rod-shaped and formed bright yellow colonies. Optimal growth occurred at 20–25 °C, pH 6–6.5, and in the presence of 0.5 % NaCl. The major fatty acids of TLA-22 were C and Cω6. The predominant hydroxy fatty acids were C 2-OH and C 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sphingoglycolipid, an unidentified aminophospholipid, an unidentified phospholipid and three unidentified lipids. TLA-22 contained spermidine as the major polyamine and putrescine as the minor component. The only isoprenoid quinone was Q-10. The genomic DNA G+C content of TLA-22 was 63.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that TLA-22 was a mem,ber of a phylogenetic lineage including members of the genus . TLA-22 was most closely related to RW16, with a 97.4 % 16S rRNA gene sequence similarity. TLA-22 showed 74.8–75.7 % average nucleotide identity and 20.1–22.0 % digital DNA–DNA hybridization identity with the strains of other species of the genus . On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TLA-22 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TLA-22 (=BCRC 81097 =LMG 30309=KCTC 62189).

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2019-10-09
2019-10-22
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