1887

Abstract

A Gram-stain-positive bacterial strain, designated LMM-1653, was isolated from a uterus swab from a Holstein Frisian dairy cow in the frame of a clinical sampling trial. The isolated strain, which showed a rod to coccoid shape, was catalase-positive and oxidase-negative. Based on 16S rRNA gene sequence similarity, its closest relatives were and (96.50 % similarity each), suggesting that this isolate represents a novel species. Strain LMM-1653 had a quinone system consisting mainly of menaquinones MK-8(H) and MK-9(H). The polar lipid profile showed presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside as well as one unidentified glycolipid and one unidentified aminoglycolipid. Moderate to minor amounts of three unidentified glycolipids, β-gentiobiosyl diacylglycerol, one unidentified aminoglycolipid and three unidentified lipids without a functional group were also found. The cell wall contained meso-diaminopimelic acid and the strain also contained corynemycolic acids. The fatty acid profile was predominantly composed of straight-chain, saturated and mono-unsaturated fatty acids, dominated by Cω9 and C. Since this isolate differs from the nearest related established species in its genetic and phenotypic traits, a novel species named LMM-1653 (=LMG-31164=CCM 8952) of the genus is proposed.

Keyword(s): Corynebacterium , endometritis and bovine
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2019-10-04
2019-10-23
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