1887

Abstract

Two novel , designated strains NEAU-YY421 and NEAU-YY642, were isolated from the rhizosphere soil of wheat ( L.) collected from Zhumadian, Henan Province, PR China and characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains NEAU-YY421 and NEAU-YY642 belonged to the genus and strain NEAU-YY421 was most closely related to CGMCC 4.1732 (97.9 % sequence similarity) and DSM 40282 (97.8 %), and that of strain NEAU-YY642 to LZS-5 (98.0 %) and YIM 65188 (97.5 %). The cell walls of the two strains contained -diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were glucose and ribose. Multilocus sequence analysis using the concatenated sequences of the , , , and genes showed that the two strains formed separate branches in the genus . Moreover, a combination of DNA–DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Therefore, strains NEAU-YY421 and NEAU-YY642 belong to two novel species in the genus , for which the names sp. nov. (NEAU-YY421=CGMCC 4.7476=DSM 106775) and sp. nov. (NEAU-YY642=CCTCC AA 2018092=DSM 107172) are proposed, respectively.

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/content/journal/ijsem/10.1099/ijsem.0.003727
2019-10-15
2019-11-12
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