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Volume 70, Issue 1

sp. nov., a novel actinomycete isolated from the ear of wheat ( L.) Free

Abstract

A novel actinomycete, designated strain NEAU-HEGS1-5, was isolated from the ear of wheat ( L.) and characterized using a polyphasic approach. The morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus . The results of 16S rRNA gene sequence analysis showed that the isolate was most closely related to NEAU-TX2-2 (99.3 %), 2C-HV3 (99.2 %), JCM 3021 (99.1 %) and subsp. JCM 3006 (98.5 %). However, two tree-making algorithms supported the position that strain NEAU-HEGS1-5 formed a distinct clade with NEAU-TX2-2, 2C-HV3 and subsp JCM 3006. Furthermore, multilocus sequence analysis based on the 16S- - genes and a combination of DNA–DNA hybridization results and some physiological and biochemical properties demonstrated that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-HEGS1-5 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-HEGS1-5 (=CCTCC AA 2019030=DSM 104648).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA gene , Microbispora fusca sp. nov. and polyphasic analysis
Funding
This study was supported by the:
  • The University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (Award UNPYSCT-2017017)
© 2020 The Authors
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2020-02-03
2023-02-01
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Microbispora fusca sp. nov., a novel actinomycete isolated from the ear of wheat (Triticum aestivum L.)
Int J Syst Evol Microbiol 70, 139 (2020); https://doi.org/10.1099/ijsem.0.003725
/content/journal/ijsem/10.1099/ijsem.0.003725
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