1887

International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 1

sp. nov., a novel actinomycete isolated from the ear of wheat ( L.) No Access

Abstract

A novel actinomycete, designated strain NEAU-HEGS1-5, was isolated from the ear of wheat ( L.) and characterized using a polyphasic approach. The morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus . The results of 16S rRNA gene sequence analysis showed that the isolate was most closely related to NEAU-TX2-2 (99.3 %), 2C-HV3 (99.2 %), JCM 3021 (99.1 %) and subsp. JCM 3006 (98.5 %). However, two tree-making algorithms supported the position that strain NEAU-HEGS1-5 formed a distinct clade with NEAU-TX2-2, 2C-HV3 and subsp JCM 3006. Furthermore, multilocus sequence analysis based on the 16S- - genes and a combination of DNA–DNA hybridization results and some physiological and biochemical properties demonstrated that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-HEGS1-5 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-HEGS1-5 (=CCTCC AA 2019030=DSM 104648).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA gene , Microbispora fusca sp. nov. and polyphasic analysis
Funding
This study was supported by the:
  • , The University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province , (Award UNPYSCT-2017017)
© 2020 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003725
2020-02-03
2021-01-20
Loading full text...

Full text loading...

References

  1. Nonomura H, Ohara Y. Distribution of actinomycetes in soil. II. Microbispora, a new genus of the Streptomycetaceae . J Ferment Technol 1957; 35:307–311
     [Google Scholar]
  2. Han C, Tian Y, Zhao J, Yu Z, Jiang S et al. Microbispora triticiradicis sp. nov., a novel actinomycete isolated from the root of wheat (Triticum aestivum L.). Int J Syst Evol Microbiol 2018; 68:3600–3605 [CrossRef]
     [Google Scholar]
  3. Han C, Zhao J, Yu B, Shi H, Zhang C et al. Microbispora tritici sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.). Antonie van Leeuwenhoek 2019; 112:11371145 [CrossRef]
     [Google Scholar]
  4. Berendsen RL, Pieterse CMJ, Bakker PAHM. The rhizosphere microbiome and plant health. Trends Plant Sci 2012; 17:478–486 [CrossRef]
     [Google Scholar]
  5. Bulgarelli D, Schlaeppi K, Spaepen S, Ver Loren van Themaat E, Schulze-Lefert P. Structure and functions of the bacterial microbiota of plants. Annu Rev Plant Biol 2013; 64:807–838 [CrossRef]
     [Google Scholar]
  6. Wang X, Zhao J, Liu C, Wang J, Shen Y et al. Nonomuraea solani sp. nov., an actinomycete isolated from eggplant root (Solanum melongena L.). Int J Syst Evol Microbiol 2013; 63:2418–2423 [CrossRef]
     [Google Scholar]
  7. Atlas RM. Handbook of microbiological media. In Parks LC. editor Microbiology Boca Raton: CRC Press; 1993
     [Google Scholar]
  8. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [CrossRef]
     [Google Scholar]
  9. Jin L, Zhao Y, Song W, Duan L, Jiang S et al. Streptomyces inhibens sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.). Int J Syst Evol Microbiol 2019; 69:688–695 [CrossRef]
     [Google Scholar]
  10. Waksman SA. The Actinomycetes A summary of current knowledge, New York: Ronald; 1967
     [Google Scholar]
  11. Jones KL. Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 1949; 57:141–145
     [Google Scholar]
  12. Waksman SA. The Actinomycetes, vol. 2, Classification, Identification and Descriptions of Genera and Species Baltimore: Williams and Wilkins; 1961
     [Google Scholar]
  13. Kelly KL. Inter-society color council-national Bureau of standards color-name charts illustrated with centroid colors published in US; 1964
  14. Fu Y, Yan R, Liu D, Jiang S, Cui L et al. Trinickia diaoshuihuensis sp. nov., a plant growth promoting bacterium isolated from soil. Int J Syst Evol Microbiol 2019; 69:291–296 [CrossRef]
     [Google Scholar]
  15. Smibert RM, Krieg NR. Phenotypic characterisation. In Gerhardt P, R. G. E Murray, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology American Society for Microbiology; 1994 pp 607–654
     [Google Scholar]
  16. Gordon RE, Barnett DA, Handerhan JE, Pang CHN. Nocardia coeliaca, Nocardia autotrophica, and the Nocardin strain. Int J Syst Bacteriol 1974; 24:54–63 [CrossRef]
     [Google Scholar]
  17. Yokota A, Tamura T, Hasegawa T, Huang LH. Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol 1993; 43:805–812 [CrossRef]
     [Google Scholar]
  18. McKerrow J, Vagg S, McKinney T, Seviour EM, Maszenan AM et al. A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 2000; 30:178–182 [CrossRef]
     [Google Scholar]
  19. Lechevalier MP, Lechevalier HA. The chemotaxonomy of actinomycetes. In Dietz A, Thayer DW. (editors) Actinomycete Taxonomy Special Publication 6 Society of Industrial Microbiology; 1980 pp 227–291
     [Google Scholar]
  20. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [CrossRef]
     [Google Scholar]
  21. Collins MD. Isoprenoid quinone analyses in bacterial classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp 267–284
     [Google Scholar]
  22. Wu C, Lu X, Qin M, Wang Y, Ruan J. Analysis of menaquinone compound in microbial cells by HPLC. Int J Syst Evol Microbiol 1989; 16:176–178
     [Google Scholar]
  23. Qu Z, Ruan JS, Hong K. Application of high performance liquid chromatography and gas chromatography in the identification of Actinomyces. Bio Bulletin 2009; s1:79–82
     [Google Scholar]
  24. Gao R, Liu C, Zhao J, Jia F, Yu C et al. Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora . Antonie van Leeuwenhoek 2014; 105:307–315 [CrossRef]
     [Google Scholar]
  25. Xiang W, Liu C, Wang X, Du J, Xi L et al. Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 2011; 61:1165–1169 [CrossRef]
     [Google Scholar]
  26. Kim SB, Brown R, Oldfield C, Gilbert SC, Iliarionov S et al. Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 2000; 50:2031–2036 [CrossRef]
     [Google Scholar]
  27. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [CrossRef]
     [Google Scholar]
  28. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [CrossRef]
     [Google Scholar]
  29. Kumar S, Stecher G, Tamura K. Mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [CrossRef]
     [Google Scholar]
  30. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [CrossRef]
     [Google Scholar]
  31. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [CrossRef]
     [Google Scholar]
  32. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [CrossRef]
     [Google Scholar]
  33. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [CrossRef]
     [Google Scholar]
  34. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4:184–192 [CrossRef]
     [Google Scholar]
  35. Savi DC, Aluizio R, Galli-Terasawa L, Kava V, Glienke C. 16S-gyrB-rpoB multilocus sequence analysis for species identification in the genus Microbispora . Antonie van Leeuwenhoek 2016; 109:801–815 [CrossRef]
     [Google Scholar]
  36. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on systematic bacteriology. Report of the ad HOC Committee on reconciliation of approaches to bacterial Systematics. Int J Syst Bacteriol 1987; 37:463–464
     [Google Scholar]
  37. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20:265–272 [CrossRef]
     [Google Scholar]
  38. Li R, Li Y, Kristiansen K, Wang J. Soap: short oligonucleotide alignment program. Bioinformatics 2008; 24:713–714 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003725
Loading
Microbispora fusca sp. nov., a novel actinomycete isolated from the ear of wheat (Triticum aestivum L.)
Int J Syst Evol Microbiol 70, 139 (2020); https://doi.org/10.1099/ijsem.0.003725
/content/journal/ijsem/10.1099/ijsem.0.003725
/content/journal/ijsem/10.1099/ijsem.0.003725
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error