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Volume 70, Issue 1

sp. nov., a novel actinobacterium isolated from a karst cave sediment sample Free

Abstract

A novel actinobacterial strain, designated SYSU K10008, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of the strain were aerobic, Gram-stain-positive and non-motile. On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008 was most closely related to CGMCC 4.1671, and shared the highest sequence identity of 98.3 % based on the NCBI database. In addition, -diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan. The whole-cell sugars were glucose and rhamnose. The major isoprenoid quinone was MK-9(H), while the major fatty acids (>10 %) were C, iso-C, anteiso-C and summed feature 3 (C 7/C 6). The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid. The genomic DNA G+C content of strain SYSU K10008 was 70.5 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU K10008 (=KCTC 39850=DSM 104115).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): karst cave , polyphasic taxonomy , sediment sample and Streptomyces cavernae sp. nov.
Funding
This study was supported by the:
  • Natural Science Foundation of Guangdong Province (Award 2016A030312003)
    • Principle Award Recipient: Wen-Jun Li
  • National Natural Science Foundation of China (Award 31600015)
    • Principle Award Recipient: Bao-Zhu Fang
  • National Key R&D Program of China (Award 2017YFD0200503)
    • Principle Award Recipient: Wen-Jun Li
© 2020 Sun Yat-Sen University, P.R. China
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2020-02-03
2024-04-19
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Streptomyces cavernae sp. nov., a novel actinobacterium isolated from a karst cave sediment sample
Int J Syst Evol Microbiol 70, 120 (2020); https://doi.org/10.1099/ijsem.0.003724
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