1887

Abstract

A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach. Strain 21Sc5-5 grew at 10–37 °C (optimum, 28–30 °C), pH 6.0–9.0 (optimum, pH 7.0) and in the presence of 0–3 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5 formed a distinct lineage within the genus and had the highest similarity to THG-S11.7 (97.30 %), followed by A2-4 (97.22 %), NSP 41 (97.15 %) and MN8 (97.15 %). The results of chemotaxonomic analyses showed that the isolate possessed -diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H) as the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids. The major fatty acids were iso-C and 10-methyl C. The genome length of strain 21Sc5-5 was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%. On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 21Sc5-5 (=JCM 33365=CGMCC 4.7608).

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2019-09-19
2019-10-19
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