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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 12

sp. nov., an endophytic plant growth promoting bacterium isolated from wheat ( subsp) in the Yaqui Valley, Mexico Free

Abstract

Strain TE3, an endophytic plant growth promoting bacterium, was isolated from wheat ( subsp. ) sampled in the Yaqui Valley, Mexico. Biochemical, phenotypic and genotypic approaches were used to clarify the taxonomic affiliation of this strain. Based on analysis of its full-length 16S rRNA gene, strain TE3 was assigned to the genus (similarity ≥98.7 %). This finding was supported by morphological and metabolic characteristics, such as rod shape, strictly aerobic metabolism, spore formation, Gram-positive staining, catalase-positive activity, reduction of nitrate to nitrite, starch and casein hydrolysis, growth in presence of lysozyme and 2 % NaCl, citrate utilization, growth pH from 6.0 to 8.0, and acid and indole production from glucose and tryptophan, respectively. The whole-genome phylogenetic relationship showed that TE3 formed an individual clade with KCTC 13622, distant from that generated by all subspecies. The maximum values for average nucleotide identity and DNA–DNA hybridization were 93.85 and 54.30 %, respectively, related to subsp. KCTC 13429. Analysis of its fatty acid content showed the ability of strain TE3 to bio-synthetize fatty acids that are not present in closely related species, such as C, C 2OH, C 3OH, C, iso-C 3OH and Cω9. These results provide evidence that strain TE3 is a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is TE3 (CM-CNRG TB54=CCStamb A1).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Average NucleotideIdentity (ANI) , Biological Control Agent , Genome-to-GenomeDistance Calculator (GGDC) and whole-genome phylogenetic
© 2019 The Authors
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2019-12-01
2024-04-19
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Bacillus cabrialesii sp. nov., an endophytic plant growth promoting bacterium isolated from wheat (Triticum turgidum subsp. durum) in the Yaqui Valley, Mexico
Int J Syst Evol Microbiol 69, 3939 (2019); https://doi.org/10.1099/ijsem.0.003711
/content/journal/ijsem/10.1099/ijsem.0.003711
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