@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003710, author = "Zhao, Yi-Ting and Li, Ya-Ping and Xiao, Wei and Liu, Wei-Hong and Cui, Xiao-Long and Wang, Yong-Xia", title = "Lampropedia aestuarii sp. nov., isolated from the estuary of a freshwater river", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "1", pages = "65-70", doi = "https://doi.org/10.1099/ijsem.0.003710", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003710", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "estuary", keywords = "polyphasic taxonomy", keywords = "Lampropedia aestuarii sp. nov.", abstract = "A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain YIM MLB12T, was isolated from estuary sediment sampled at Maliao River where it flows into a plateau lake (Dianchi) in Yunnan, south-west PR China. Cells were non-motile and rod-shaped. Growth was observed at 15–35 °C (optimum, 25–30 °C), pH 6.0–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7 % (w/v) NaCl (optimum, 0.5–2 %). Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM MLB12T formed a tight phylogenic lineage with members of the genus Lampropedia and was closely related to ‘ Lampropedia puyangensis ’ 2-bin with 98.3 % sequence similarity and had low similarities to the type strains of Lampropediahyalina ATCC 11041T (96 %) and Lampropedia cohaerens CT6T (95.5 %). Average nucleotide identity and in silico DNA–DNA hybridization values between strain YIM MLB12T and ‘ L. puyangensis ’ KCTC 32235 were 76.5 and 22.6 %, respectively. Strain YIM MLB12T contained ubiquinone-8 as the major quinone. The predominant cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0, C10 : 0 3-OH, summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c), C12 : 0 3-OH and C14 : 0. The polar lipid profile of strain YIM MLB12T was composed predominantly of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The major polyamine was spermidine. The genomic DNA G+C content of strain YIM MLB12T was 56.8 mol%. Based on its genotypic and chemotaxonomic features and results of phenotypic analyses, strain YIM MLB12T represents a novel species of the genus Lampropedia , for which the name Lampropedia aestuarii sp. nov. is proposed. The type strain is YIM MLB12T (=KCTC 42886T=CGMCC 1.17071T).", }