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Volume 70, Issue 1

sp. nov., isolated from the estuary of a freshwater river Open Access

Abstract

A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain YIM MLB12, was isolated from estuary sediment sampled at Maliao River where it flows into a plateau lake (Dianchi) in Yunnan, south-west PR China. Cells were non-motile and rod-shaped. Growth was observed at 15–35 °C (optimum, 25–30 °C), pH 6.0–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–7 % (w/v) NaCl (optimum, 0.5–2 %). Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM MLB12 formed a tight phylogenic lineage with members of the genus and was closely related to ‘’ 2-bin with 98.3 % sequence similarity and had low similarities to the type strains of ATCC 11041 (96 %) and CT6 (95.5 %). Average nucleotide identity and DNA–DNA hybridization values between strain YIM MLB12 and ‘’ KCTC 32235 were 76.5 and 22.6 %, respectively. Strain YIM MLB12 contained ubiquinone-8 as the major quinone. The predominant cellular fatty acids were summed feature 3 (C ω7 and/or C ω6), C, C 3-OH, summed feature 8 (C ω6 and/or C ω7), C 3-OH and C. The polar lipid profile of strain YIM MLB12 was composed predominantly of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The major polyamine was spermidine. The genomic DNA G+C content of strain YIM MLB12 was 56.8 mol%. Based on its genotypic and chemotaxonomic features and results of phenotypic analyses, strain YIM MLB12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM MLB12 (=KCTC 42886=CGMCC 1.17071).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): estuary , Lampropedia aestuarii sp. nov. and polyphasic taxonomy
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Lampropedia aestuarii sp. nov., isolated from the estuary of a freshwater river
Int J Syst Evol Microbiol 70, 65 (2020); https://doi.org/10.1099/ijsem.0.003710
/content/journal/ijsem/10.1099/ijsem.0.003710
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