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Volume 70, Issue 1

sp. nov., isolated from the Free

Abstract

Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410 and 553, were isolated from faeces of the Tibetan antelope () from the Tibet-Qinghai Plateau, PR China. The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7. The end products from glucose fermentation included ethanol and lactic acid. Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410 and 553 were classified into the genus , and were closely related to (97.6 %), (93.5 %) and (90.8 %). The genomic G+C content of strain 410 was 67.4 mol%. Digital DNA–DNA hybridization values between strain 410 and each of the closely related species were under 70 %. The respiratory quinones were MK-10 (68 %) and MK-9 (32 %). The main cellular fatty acids of the isolates were C, followed by C 9. The major polar lipids were diphosphatidylglycerol and phosphatidylinositol-mannoside. The whole-cell sugars contained rhamnose, ribose and glucose. The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine. The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus , for which the name s sp. nov. is proposed. The type strain is 410 (=CGMCC 1.16361= DSM 106201).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomyces qiguomingii sp. nov. , Qinghai-Tibet Plateau and Tibetan antelope
Funding
This study was supported by the:
  • National Key R&D Program of China (Award 2018YFC1200102)
    • Principle Award Recipient: Dong Jin
  • National Science and Technology Major Project of China (Award 2018ZX10305409-003)
    • Principle Award Recipient: Zhihong Ren
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: Jing Yang
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Jianguo Xu
© 2020 The Authors
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2019-10-14
2024-04-25
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Actinomyces qiguomingii sp. nov., isolated from the Pantholops hodgsonii
Int J Syst Evol Microbiol 70, 58 (2020); https://doi.org/10.1099/ijsem.0.003709
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