A yellow, Gram-stain-negative, aerobic, non-gliding, non-spore-forming, rod-shaped strain, designated YIM 102600T, was isolated from the faeces of Macaca mulatta dwelling in the Yunnan Wild Animal Park, Yunnan Province, South-West PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 102600T was a member of the genus Flavobacterium, and closely related to Flavobacterium qiangtangense F3T (96.9 % similarity) and Flavobacterium noncentrifugens R-HLS-17T (96.0 % similarity). Phylogenetic trees showed that strain YIM 102600T formed a clade with F. qiangtangense F3T and F. noncentrifugens R-HLS-17T. Growth occurred at 4–30 °C (optimum, 28 °C), pH 7.0–8.0 (pH 7.5) and NaCl concentration 0–2 % (w/v; 0–1 %, w/v). The major fatty acids were iso-C15:0 and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c). The predominant polar lipid was phosphatidylethanolamine and the sole respiratory quinone was menaquinone-6. The DNA G+C content was 36.4 mol%. The calculated digital DNA–DNA hybridization values between strain YIM 102600T and other species of Flavobacterium ranged from 70.0 to 75.0 % and average nucleotide identity values were in a range between 13.7 to 23.5 %. Based above the consensus of phenotypic and phylogenetic analyses as well as whole genome comparisons, strain YIM 102600T (=KCTC 52099T=CCTCC AB 201632T) is proposed to represent type strain of a novel species, Flavobacterium macacae sp. nov.
HolmesB,
SnellJJS,
LapageSP.
Revised description, from clinical strains, of Flavobacterium breve (Lustig) Bergey et al. 1923 and proposal of the neotype strain. Int J Syst Bacteriol1978; 28:201–208 [View Article]
BernardetJF,
SegersP,
VancanneytM,
BertheF,
KerstersK et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis STROHL and Tait 1978). Int J Syst Bacteriol1996; 46:128–148 [View Article]
KangJY,
ChunJ,
JahngKY.
Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium
. Int J Syst Evol Microbiol2013; 63:1633–1638 [View Article]
BernardetJF,
BowmanJP.
The genus Flavobacterium
. In
Dworkin MS,
Falkow SE,
RosenbergE,
SchleiferKH,
StackebrandtE.
(editors) The Prokaryotes: a Handbook on the Biology of Bacteria7, 3nd edn. 2006 pp 481–531
BernardetJF,
NakagawaY,
HolmesB.
Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol2002; 52:1049–1070
SmibertRM,
KriegNR.
Phenotypic characterization. In
GerhardtP,
MurrayRGE,
WoodWA,
KriegNR.
(editors) Methods for General and Molecular Bacteriology. American Society for Microbiology Washington: DC; 1994 pp 607–654
CernyG.
Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. European J. Appl. Microbiol. Biotechnol.1978; 5:113–122 [View Article]
XuP,
LiW-J,
TangS-K,
ZhangY-Q,
ChenG-Z et al.Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol2005; 55:1149–1153 [View Article]
TindallBJ,
SikorskiJ,
SmibertRA,
KriegNR.
Phenotypic characterization and the principles of comparative systematics. In
ReddyCA,
BeveridgeTJ,
BreznakJA,
MarzlufGA,
SchmidtTM.
(editors) Methods for General and Molecular Microbiology. American Society for Microbiology Washington: DC; 2007 pp 330–393
ReichenbachH,
OrderI,
LeadbetterC.
Order I. Cytophagales Leadbetter 1974. In
StaleyJT,
BryantMP,
PfennigN,
HoltJG.
(editors) In Bergey’s Manual of Systematic Bacteriology3 Baltimore: Williams & Wilkins; 1989 pp 2011–2013
CollinsMD,
GoodfellowM,
MinnikinDE.
Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol1980; 118:29–37 [View Article]
CollinsMD.
Isoprenoid quinone analysis in classification and identification. In
GoodfellowM,
MinnikinDE.
(editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp 267–287
WuC,
LuX,
QinM,
WangY,
RuanJ.
Analysis of menaquinone compound in microbial cells by HPLC. Microbiology English translation of Microbiology, Beijing1989; 16:176–178
LiW-J,
XuP,
SchumannP,
ZhangY-Q,
PukallR et al.Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia
. Int J Syst Evol Microbiol2007; 57:1424–1428 [View Article]
CuiXL,
MaoPH,
ZengM,
LiWJ,
ZhangLP et al.Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae
. Int J Syst Evol Microbiol2001; 51:357–363 [View Article]
DHL,
LiuCM,
LuoRB,
SadakaneKH,
LamTW.
MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics2015; 31:1674–1676
MesbahM,
PremachandranU,
WhitmanWB.
Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol1989; 39:159–167 [View Article]
YoonS-H,
HaS-M,
LimJ,
KwonS,
ChunJ.
A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek2017; 110:1281–1286 [View Article]
Meier-KolthoffJP,
GökerM,
SpröerC,
KlenkH-P.
When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol2013; 195:413–418 [View Article]
WayneLG,
BrennerDJ,
ColwellRR,
GrimontPAD,
KandlerO et al. International Committee on systematic bacteriology. Report of the AD Hoc Committee on reconciliation of approaches to bacterial Systematics. Int J Syst Evol Microbiol1987; 37:463–464