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Volume 69, Issue 12

sp. nov., isolated from a sulfonylurea herbicide-degrading consortium enriched with saline soil Free

Abstract

A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated strain LAM9072, was isolated from a sample of a sulfonylurea herbicide-degrading consortium enriched with saline soil. The optimal temperature and pH for the growth of strain LAM9072 were 35 °C and 7.0, respectively. Strain LAM9072 could grow in the presence of NaCl up to 9 % (w/v). Comparative analysis of the 16S rRNA gene sequences revealed that strain LAM9072 was closely related to members of the family , with the highest similarities to MACL01 (97.7 %) and S2753 (97.7 %). Strain LAM9072 formed a distinct phylogenetic subclade within the genus in the 16S rRNA gene phylogenetic trees. The results of multi-locus sequence analysis revealed a distinct lineage with MACL01 as its closest relative. The genomic G+C content was 50.2 mol%. The DNA–DNA hybridization values between strain LAM9072 and LMG 22194 and LMG 28894 were 41.6 and 22.2 %, respectively. The average nucleotide identity values were 90.9 and 78.8 %, respectively, by comparing the draft genome sequences of strain LAM9072 and LMG 22194 and LMG 28894. The major fatty acids were summed feature 3 (Cω6 and/or Cω7), C and summed feature 8 (Cω7 and/or Cω6). Ubiquinone 8 was detected as the predominant respiratory quinone. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and four unidentified lipids. Based on its phenotypic characteristics and the results of genotypic analyses, we propose that strain LAM9072 represents a novel species, for which the name sp. nov. is proposed. The type strain is LAM9072 (=ACCC 19961=JCM 30852).

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA gene , Photobacterium , polyphasic taxonomy and saline soil
© 2019 The Authors
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2019-12-01
2024-04-25
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Photobacterium salinisoli sp. nov., isolated from a sulfonylurea herbicide-degrading consortium enriched with saline soil
Int J Syst Evol Microbiol 69, 3910 (2019); https://doi.org/10.1099/ijsem.0.003705
/content/journal/ijsem/10.1099/ijsem.0.003705
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