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Volume 69, Issue 12

sp. nov., isolated from activated sludge Free

Abstract

A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped and aerobic bacterial strain (designated lm1) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong province, PR China). Strain lm1 grew between 15 and 45 °C (optimum, 40 °C). The pH range for growth was at pH 4.0–10.0 (optimum growth at pH 8.0). The range of NaCl concentration for the growth of strain lm1 was 0–4.0 % (w/v), with optimum growth at 1.0–2.0 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain lm1 belonged to the genus and was closely related to JCM 16873 (96.7 % 16S rRNA gene sequence similarity) and CCTCC AB2013083 (96.2 %). The average nucleotide identity value and the digital DNA–DNA hybridization value between strain lm1 and JCM 16873 were 73.4 and 14.3 %, respectively. The major cellular fatty acids of strain lm1 were Ccyclo ω8, Cω7 and C. Strain lm1 contained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and five unknown lipids. The G+C content of the genomic DNA was 64.4 mol%. Based on polyphasic taxonomic data, strain lm1 could be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is lm1 (=CCTCC AB2019071=KCTC 72156).

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  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , phylogenetic analysis and Rhodoligotrophos
© 2019 The Authors
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2019-12-01
2024-04-19
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Rhodoligotrophos defluvii sp. nov., isolated from activated sludge
Int J Syst Evol Microbiol 69, 3830 (2019); https://doi.org/10.1099/ijsem.0.003691
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