1887

Abstract

A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped and aerobic bacterial strain (designated lm1) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong province, PR China). Strain lm1 grew between 15 and 45 °C (optimum, 40 °C). The pH range for growth was at pH 4.0–10.0 (optimum growth at pH 8.0). The range of NaCl concentration for the growth of strain lm1 was 0–4.0 % (w/v), with optimum growth at 1.0–2.0 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain lm1 belonged to the genus and was closely related to JCM 16873 (96.7 % 16S rRNA gene sequence similarity) and CCTCC AB2013083 (96.2 %). The average nucleotide identity value and the digital DNA–DNA hybridization value between strain lm1 and JCM 16873 were 73.4 and 14.3 %, respectively. The major cellular fatty acids of strain lm1 were Ccyclo ω8, Cω7 and C. Strain lm1 contained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and five unknown lipids. The G+C content of the genomic DNA was 64.4 mol%. Based on polyphasic taxonomic data, strain lm1 could be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is lm1 (=CCTCC AB2019071=KCTC 72156).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003691
2019-09-10
2019-10-18
Loading full text...

Full text loading...

References

  1. Fukuda W, Yamada K, Miyoshi Y, Okuno H, Atomi H et al. Rhodoligotrophos appendicifer gen. nov., sp. nov., an appendaged bacterium isolated from a freshwater Antarctic lake. Int J Syst Evol Microbiol 2012;62:1945–1950 [CrossRef][PubMed]
    [Google Scholar]
  2. Deng SK, Chen GQ, Chen Q, Cai S, Yao L et al. Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 2014;64:3325–3330 [CrossRef][PubMed]
    [Google Scholar]
  3. Mu DS, Liang QY, Wang XM, Lu DC, Shi MJ et al. Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 2018;6:230 [CrossRef][PubMed]
    [Google Scholar]
  4. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68:461–466 [CrossRef][PubMed]
    [Google Scholar]
  5. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014;64:2204–2209 [CrossRef][PubMed]
    [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  7. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  10. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  11. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000;50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  12. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and cytophaga-like bacteria of the international committee on systematics of prokaryotes proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070
    [Google Scholar]
  13. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  14. Cowan ST, Steel KJ, Characters B. Characterization, 2nd ed. Cambridge, UK: Cambridge University Press; 1974
    [Google Scholar]
  15. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014;64:1690–1696 [CrossRef][PubMed]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  17. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982;5:2359–2367 [CrossRef]
    [Google Scholar]
  18. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  19. Xu XW, Wu YH, Wang CS, Oren A, Zhou PJ et al. Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 2007;57:717–720 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003691
Loading
/content/journal/ijsem/10.1099/ijsem.0.003691
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error