@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003687, author = "Zhang, Guo-Qing and Liu, Qing and Liu, Hong-Can and Zhou, Yu-Guang and Xin, Yu-Hua", title = "Flavobacterium ranwuense sp. nov., isolated from glacier", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "12", pages = "3812-3817", doi = "https://doi.org/10.1099/ijsem.0.003687", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003687", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Flavobacterium ranwuense", keywords = "psychrotolerant", keywords = "glacier", abstract = "A Gram-stain-negative, aerobic, rod-shaped, devoid of flagella, orange–yellow-coloured bacterium (strain LB2P22T) was isolated from an ice sample collected from the Laigu glacier on the Tibetan Plateau, PR China. The results of 16S rRNA gene sequence analysis indicated that strain LB2P22T belongs to the genus Flavobacterium with highest similarity to Flavobacterium laiguense LB2P30T (98.14 %), Flavobacterium glaciei CGMCC 1.5380T (98.02 %), Flavobacterium psychroterrae CCM 8827T (97.94 %) and Flavobacterium granuli CGMCC 1.10125T (97.91 %). Strain LB2P22T had 77.99–84.99 % average nucleotide identity and 22.3–29.3 % digital DNA–DNA hybridization values with its closest relatives, indicating that it represents a novel species of the genus Flavobacterium . Strain LB2P22T grew at 0–25 °C, pH 7.0–8.0 and up to 1.0 % (w/v) NaCl. The main cellular fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c) and anteiso-C15 : 0. The genomic DNA G+C content is 34.3 mol%. The predominant menaquinone was MK-6 and the polar lipids were phosphatidylethanolamine, one unidentified aminolipid and four unidentified lipids. Based on these data, a novel species, Flavobacterium ranwuense sp. nov., is proposed, with LB2P22T (=CGMCC 1.11361T=NBRC 113777T) as the type strain.", }