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Volume 69, Issue 12

sp. nov., a xylan-degrading bacterium isolated from decaying forest soil Free

Abstract

A xylanolytic bacterial strain, named A59, was isolated from a forest soil consortium in southern Argentina. Strain A59 is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its optimal growth conditions are 30 °C (range, 28–37 °C), pH 7 (range, pH 5–10) and it tolerates up to 7 % of NaCl (range, 2–7 %). Chemotaxonomic analysis revealed that strain A59possesses -diaminopimelic acid in the cell wall. It contains menaquinone MK-7 as the predominant isoprenoid quinone and the major fatty acid is anteiso-C (35.1 %), with a moderate amount of C (6.9 %). According to 16S RNA gene sequence analysis, the isolate is phylogenetically placed in the same cluster as BCRC 17757 (99.7 % nucleotide sequence identity) and NBRC 13638 (99.1 %) and is closely related to A10b (98.8 %). However, phylogenetic studies based on the housekeeping gene placed A59 in a separate branch from all other related type strains. Furthermore, the results of whole genome average nucleotide identity analysis (gANI) with related type strains was lower than 91.10 % and the digital DNA–DNA hybridization value was lower than 44.30 %, which are below the threshold values for separating two species. The DNA G+C content was estimated as 46.09 mol%, based on genome sequencing. On the basis of these results, A59 represents a new species of the genus , and we propose the name sp. nov. The type strain is A59 (=DSM 107920=NCIMB 15123).

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Keyword(s): bacteria , forest soil , Paenibacillus xylanivorans and xylanase
© 2019 The Authors
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2019-12-01
2024-04-19
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Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil
Int J Syst Evol Microbiol 69, 3818 (2019); https://doi.org/10.1099/ijsem.0.003686
/content/journal/ijsem/10.1099/ijsem.0.003686
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