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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 12

sp. nov., isolated from farmland soil Free

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped, yellow, non-spore-forming bacterial strain, designated P-25, was isolated from soil collected in Yantai, Shandong Province, PR China. The temperature, pH and NaCl concentration ranges for the growth of strain P-25 were 10–37 °C (optimum, 28–30 °C), pH 6.0–9.0 (optimum, pH 7.5–8.0) and 0–4 % (w/v) (optimum, 1 % w/v), respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain P-25 was most closely related to S27 (98.1 % 16S rRNA gene sequence similarity), followed by CM134L-2 (97.2 %) and THG-T11 (97.1 %). The genomic DNA G+C content of strain P-25 based on its draft genome sequence was 38.1 %. MK-7 was the major respiratory quinone, and iso-C, Cω7 and/or Cω6 (summed feature 3) and iso-C 3-OH were the major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, two unidentified lipids, five unidentified aminolipids and two unidentified glycolipids. Average nucleotide identity values for the draft genomes between strain P-25 and strains S27, CM134L-2 and THG-T11 were 81.8, 77.6 and 81.2 %, respectively, and the digital DNA–DNA hybridization (dDDH) values were 30.0, 19.2 and 27.6 %, respectively. Based on their phylogenetic and phenotypic characteristics, chemotaxonomic data, and dDDH results, strain P-25 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is strain P-25 (KCTC 62821=CCTCC AB 2018185).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): farmland soil , Pedobacter helvus sp. nov. and polyphasic taxonomy
© 2019 The Authors
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Pedobacter helvus sp. nov., isolated from farmland soil
Int J Syst Evol Microbiol 69, 3806 (2019); https://doi.org/10.1099/ijsem.0.003684
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