1887

Abstract

A Gram-stain-negative, rod-shaped, non-motile, aerobic, orange-pigmented, bacterium (designated 176CP4-71) was isolated from a sponge sample of from Cheongpo beach, Taean district, in the Republic of Korea. On the basis of 16S rRNA gene sequencing, strain 176CP4-71 clustered with species of the genus and appeared to be closely related to LMG 26637 (95.4 %), JCM 18450 (94.7 %), KACC 16195 (94.7 %) and KCTC 22339 (94.2 %). The average nucleotide identity values between strain 176CP4-71 and the closely related strains were 70.7–73.7 %, indicating that 176CP4-71 is a novel species of the genus . Growth occurs at 10–35 °C on marine agar medium in the presence of 1–8 % NaCl (w/v) and at pH 6.0–8.5. The DNA G+C content of the genomic DNA was 41.6 mol %, and menaquinone with six isoprene units (MK-6) was the major respiratory quinone. The major cellular fatty acids (>5 %) were Ciso, Ciso G, Ciso 3OH and Cω6 and/or Cω7 (summed feature 3). The polar lipids consisted of phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified phospholipid and unidentified lipids. Physiological and biochemical characteristics indicated that strain 176CP4-71 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 176CP4-71 (=KACC 19889=LMG 31033).

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2019-10-23
2019-11-12
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References

  1. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001;51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  2. Yoon JH, Lee MH, Oh TK, Park YH. Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda. Int J Syst Evol Microbiol 2005;55:1015–1019 [CrossRef][PubMed]
    [Google Scholar]
  3. Hwang CY, Kim MH, Bae GD, Zhang GI, Kim YH et al. Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda. Int J Syst Evol Microbiol 2009;59:1856–1861 [CrossRef][PubMed]
    [Google Scholar]
  4. Arun AB, Chen WM, Lai WA, Chao JH, Rekha PD et al. Muricauda lutaonensis sp. nov., a moderate thermophile isolated from a coastal hot spring. Int J Syst Evol Microbiol 2009;59:2738–2742 [CrossRef][PubMed]
    [Google Scholar]
  5. Yang C, Li Y, Guo Q, Lai Q, Wei J et al. Muricauda zhangzhouensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2013;63:2320–2325 [CrossRef][PubMed]
    [Google Scholar]
  6. Liu SQ, Sun QL, Sun YY, Yu C, Sun L. Muricauda iocasae sp. nov., isolated from deep sea sediment of the South China Sea. Int J Syst Evol Microbiol 2018;68:2538–2544 [CrossRef][PubMed]
    [Google Scholar]
  7. Wang Y, Yang X, Liu J, Wu Y, Zhang XH. Muricauda lutea sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2017;67:1064–1069 [CrossRef][PubMed]
    [Google Scholar]
  8. Su Y, Yang X, Wang Y, Liu Y, Ren Q et al. Muricauda marina sp. nov., isolated from marine snow of Yellow Sea. Int J Syst Evol Microbiol 2017;67:2446–2451 [CrossRef][PubMed]
    [Google Scholar]
  9. Zhang Z, Gao X, Qiao Y, Wang Y, Zhang XH. Muricauda pacifica sp. nov., isolated from seawater of the South Pacific Gyre. Int J Syst Evol Microbiol 2015;65:4087–4092 [CrossRef][PubMed]
    [Google Scholar]
  10. Wu YH, Yu PS, Zhou YD, Xu L, Wang CS et al. Muricauda antarctica sp. nov., a marine member of the Flavobacteriaceae isolated from Antarctic seawater. Int J Syst Evol Microbiol 2013;63:3451–3456 [CrossRef][PubMed]
    [Google Scholar]
  11. Kim JM, Jin HM, Jeon CO. Muricauda taeanensis sp. nov., isolated from a marine tidal flat. Int J Syst Evol Microbiol 2013;63:2672–2677 [CrossRef][PubMed]
    [Google Scholar]
  12. Buck JD. Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 1982;44:992–993[PubMed]
    [Google Scholar]
  13. Weon HY, Kim BY, Joa JH, Son JA, Song MH et al. Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea. Int J Syst Evol Microbiol 2008;58:93–96 [CrossRef][PubMed]
    [Google Scholar]
  14. Atlas RM. Handbook of Microbiological Media Boca Raton, Florida, USA: CRC Press; 1993
    [Google Scholar]
  15. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press; 1974
    [Google Scholar]
  16. Cappuccino JG, Sherman N. A Laboratory Manual, 6th ed. CA: Pearson Education, Inc; 2002
    [Google Scholar]
  17. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics New York: Wiley; 1991
    [Google Scholar]
  18. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  19. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95–98
    [Google Scholar]
  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  21. Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  22. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  23. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425
    [Google Scholar]
  24. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  25. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  26. Tatusova T, Dicuccio M, Badretdin A, Chetvernin V, Nawrocki EP et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 2016;44:6614–6624 [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110:1281–1286 [CrossRef][PubMed]
    [Google Scholar]
  28. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996;42:457–469 [CrossRef]
    [Google Scholar]
  29. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI technical note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  30. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  31. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64:346–351 [CrossRef][PubMed]
    [Google Scholar]
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