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Volume 69, Issue 12

sp. nov., isolated from a saline lake in Inner Mongolia Free

Abstract

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterium, designated D20, was isolated from the saline Lake Dai in Inner Mongolia, PR China. Growth of strain D20 occurred at 25–45 °C (optimum, 40 °C), pH 4.0–12.0 (optimum, 8.0) and with 0–3 % NaCl (w/v); (optimum, 0–1 %). The results of 16S rRNA gene sequence analysis revealed that strain D20 was most closely related to three species, AX, S2 and K172, with a similarity value of 96.2 %. The major respiratory quinone of strain D20 was ubiquinone-8 (Q-8), and the dominant fatty acids (>10 %) were summed feature 3 (Cω6 and/or Cω7; 39.8 %), C (30.9 %) and summed feature 8 (Cω6 and/or Cω7; 13.5 %). The polar lipid profile contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one aminophospholipid and five unidentified lipids. The DNA G+C content was 67.2 mol% (data from the genome sequence). The estimated genome size was 3.7 Mb. The phenotypic, genotypic and chemotaxonomic differences between strain D20 and its phylogenetic relatives indicated that strain D20 should be regarded as a novel species in the genus , for which the name sp. nov. is proposed. The type strain is D20 (=MCCC 1H00305=KCTC 62586).

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Keyword(s): 16S rRNA gene , ANI , benzoate degradation pathway , dDDH , draft genome sequencing and Thauera
© 2019 The Authors
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2019-12-01
2024-04-25
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Thauera lacus sp. nov., isolated from a saline lake in Inner Mongolia
Int J Syst Evol Microbiol 69, 3786 (2019); https://doi.org/10.1099/ijsem.0.003679
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