Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas. Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA–DNA relatedness through DNA–DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively. Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores. The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species. The G+C content of MS2379T is 45.9 mol%. Fermentation of glucose yields acid and gas end-products. The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine. The growth range of MS2379T was observed from 10–45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6–10 and up to 3 % NaCl. Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction. Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T. Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp. nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
GradyEN,
MacDonaldJ,
LiuL,
RichmanA,
YuanZ-C.
Current knowledge and perspectives of Paenibacillus: a review. Microb Cell Fact2016; 15:1–18 [View Article]
BenardiniJN,
VaishampayanPA,
SchwendnerP,
SwannerE,
FukuiY et al.Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine. Int J Syst Evol Microbiol2011; 61:1338–1343 [View Article]
AguileraM,
Monteoliva-SánchezM,
SuárezA,
GuerraV,
LizamaC et al.Paenibacillus jamilae sp. nov., an exopolysaccharide-producing bacterium able to grow in olive-mill wastewater. Int J Syst Evol Microbiol2001; 51:1687–1692 [View Article]
PriestFG.
Genus I. Paenibacillus
. In
De VosP,
GarrityGM,
JonesD,
KriegNR,
LudwigW.
(editors) Bergey’s Manual of Systematic Bacteriology New York: Springer; 2009 pp 269–295
LoganNA,
BergeO,
BishopAH,
BusseH-J,
De VosP et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol2009; 59:2114–2121 [View Article]
da MotaFF,
GomesEA,
PaivaE,
RosadoAS,
SeldinL.
Use of rpoB gene analysis for identification of nitrogen-fixing Paenibacillus species as an alternative to the 16S rRNA gene. Lett Appl Microbiol2004; 39:34–40 [View Article]
AltschulSF,
MaddenTL,
SchäfferAA,
ZhangJ,
ZhangZ et al. Gapped blast and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res1997; 25:3389–3402 [View Article]
KimM,
OhH-S,
ParkS-C,
ChunJ,
Hyun-SeokO.
Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol2014; 64:346–351 [View Article]
ChunJ,
OrenA,
VentosaA,
ChristensenH,
ArahalDR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol2018; 68:461–466 [View Article]
OverbeekR,
OlsonR,
PuschGD,
OlsenGJ,
DavisJJ et al. The seed and the rapid annotation of microbial genomes using subsystems technology (RAST). Nucleic Acids Res2014; 42:D206–D214 [View Article]
BrettinT,
DavisJJ,
DiszT,
EdwardsRA,
GerdesS et al. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci Rep2015; 5:8365 [View Article]
Rodriguez-RLM,
KonstantinidisKT.
The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. PeerJ Preprints2016
GorisJ,
KonstantinidisKT,
KlappenbachJA,
CoenyeT,
VandammeP et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol2007; 57:81–91 [View Article]
RhulandLE,
WorkE,
DenmanRF,
HoareDS.
The behavior of the isomers of α,ε-diaminopimelic acid on paper chromatograms. J Am Chem Soc1955; 77:4844–4846 [View Article]
HussVA,
FestlH,
SchleiferKH.
Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol1983; 4:184–192 [View Article]
MesbahM,
PremachandranU,
WhitmanWB.
Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol1989; 39:159–167 [View Article]
TindallBJ,
SikorskiJ,
SmibertRA,
KriegNR.
Phenotypic characterization and the principles of comparative systematics. Methods for General and Molecular Microbiology, 3rd ed. American Society of Microbiology; 2007 pp 330–393
ShidaO,
TakagiH,
KadowakiK,
NakamuraLK,
KomagataK.
Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus
. Int J Syst Bacteriol1997; 47:289–298 [View Article]
WayneLG,
MooreWEC,
StackebrandtE,
KandlerO,
ColwellRR et al. Report of the ad hoc Committee on reconciliation of approaches to bacterial Systematics. Int J Syst Evol Microbiol1987; 37:463–464 [View Article]
BaldaniJI,
ReisVM,
VideiraSS,
BoddeyLH,
BaldaniVLD.
The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant Soil2014; 384:413–431 [View Article]
HeyndrickxM,
VandemeulebroeckeK,
ScheldemanP,
KerstersK,
de VosP et al. A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae
. Int J Syst Bacteriol1996; 46:988–1003 [View Article]