1887

Abstract

Five yeast strains were isolated from rotting wood samples collected in the Xishuangbanna Tropical Rainforest, Yunnan Province, PR China. Phylogenetic analysis of the D1/D2 domains of the large subunit rRNA gene indicated that these strains represent two novel species of the genus sp. nov. produces one to two spherical ascospores per ascus, and is most closely related to and sp. nov. differed from the type strains of the two latter species by 13–24 substitutions in the D1/D2 domains and by 39–56 substitutions in the ITS regions. f.a., sp. nov. is a member of the subclade, but the formation of ascospores was not observed on various sporulation media. sp. nov. differed from other members of the subclade by 23–26 substitutions in the D1/D2 domains and by more than 67 substitutions in the ITS regions. The holotype of sp. nov. is NYNU 17944 (CBS 15232) and the holotype of sp. nov. is NYNU 18913 (CBS 16054).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003670
2019-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/11/3623.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003670&mimeType=html&fmt=ahah

References

  1. Zubkova RD. Genus novum Saccharomycetacearume Kazachstania . Bot Mater Gerbg Inst Bot Akad Kazakh SSR 1971; 7:53–56
    [Google Scholar]
  2. Kurtzman CP. Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora . FEMS Yeast Res 2003; 4:233–245 [View Article][PubMed]
    [Google Scholar]
  3. Kurtzman CP, Robnett CJ. Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 2003; 3:417–432 [View Article][PubMed]
    [Google Scholar]
  4. Vaughan-Martini A, Lachance MA, Kurtzman CP. Kazachstania Zubkova (1971). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed. vol. 2 Amsterdam: Elsevier; 1971
    [Google Scholar]
  5. Chen R, Wei SC, Jiang YM, Wang QM, Bai FY. Kazachstania taianensis sp. nov., a novel ascomycetous yeast species from orchard soil. Int J Syst Evol Microbiol 2010; 60:1473–1476 [View Article][PubMed]
    [Google Scholar]
  6. Suh SO, Zhou JJ. Kazachstania intestinalis sp. nov., an ascosporogenous yeast from the gut of passalid beetle Odontotaenius disjunctus . Antonie van Leeuwenhoek 2011; 100:109–115 [View Article][PubMed]
    [Google Scholar]
  7. Safar SV, Gomes FC, Marques AR, Lachance MA, Rosa CA. Kazachstania rupicola sp. nov., a yeast species isolated from water tanks of a bromeliad in Brazil. Int J Syst Evol Microbiol 2013; 63:1165–1168 [View Article][PubMed]
    [Google Scholar]
  8. James SA, Carvajal Barriga EJ, Portero Barahona P, Nueno-Palop C, Cross K et al. Kazachstania yasuniensis sp. nov., an ascomycetous yeast species found in mainland Ecuador and on the Galápagos. Int J Syst Evol Microbiol 2015; 65:1304–1309 [View Article][PubMed]
    [Google Scholar]
  9. Jacques N, Sarilar V, Urien C, Lopes MR, Morais CG et al. Three novel ascomycetous yeast species of the Kazachstania clade, Kazachstania saulgeensis sp. nov., Kazachstania serrabonitensis sp. nov. and Kazachstania australis sp. nov. Reassignment of Candida humilis to Kazachstania humilis f.a. comb. nov. and Candida pseudohumilis to Kazachstania pseudohumilis f.a. comb. nov. Int J Syst Evol Microbiol 2016; 66:5192–5200 [View Article][PubMed]
    [Google Scholar]
  10. Kurtzman CP, Robnett CJ, Ward JM, Brayton C, Gorelick P et al. Multigene phylogenetic analysis of pathogenic candida species in the Kazachstania (Arxiozyma) telluris complex and description of their ascosporic states as Kazachstania bovina sp. nov., K. heterogenica sp. nov., K. pintolopesii sp. nov., and K. slooffiae sp. nov. J Clin Microbiol 2005; 43:101–111 [View Article][PubMed]
    [Google Scholar]
  11. Zheng J, Liu KF, Liu XJ, Zhang L, Hui FL et al. Deakozyma yunnanensis sp. nov., a novel yeast species isolated from rotten wood. Int J Syst Evol Microbiol 2017; 67:2436–2439 [View Article][PubMed]
    [Google Scholar]
  12. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed. vol. 1 Amsterdam: Elsevier; 2011 pp. 87–110
    [Google Scholar]
  13. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  14. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990 pp. 315–322
    [Google Scholar]
  15. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  16. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  17. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  18. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526 [View Article][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003670
Loading
/content/journal/ijsem/10.1099/ijsem.0.003670
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error