sp. nov., isolated from the junction between the ocean and a freshwater lake and emended description of the genus Free

Abstract

A novel marine Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain DSL-48, was isolated from tidal flat sediment sampled from the East China Sea and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 35 °C (range, 4–37 °C), pH 6 (pH 5–10) and with 4 % (w/v) NaCl (0–7 %). The nearest phylogenetic neighbour was KCTC 52638 with 16S rRNA gene similarity of 97.1 %. The predominant respiratory quinone was menaquinone-6 (MK-6). The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major fatty acids of strain DSL-48 were iso-C, iso-C 3-OH, anteiso-C, iso-C 3-OH and iso-C 3-OH. The G+C content of the genomic DNA was 33.3 mol%. The combined genotypic and phenotypic data indicated that strain DSL-48 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain DSL-48 (=KCTC 62648=MCCC 1K03537).

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2019-11-01
2024-03-28
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