Confluentibacter sediminis sp. nov., isolated from the junction between the ocean and a freshwater lake and emended description of the genus Confluentibacter
A novel marine Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain DSL-48T, was isolated from tidal flat sediment sampled from the East China Sea and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 35 °C (range, 4–37 °C), pH 6 (pH 5–10) and with 4 % (w/v) NaCl (0–7 %). The nearest phylogenetic neighbour was Confluentibacter citreus KCTC 52638T with 16S rRNA gene similarity of 97.1 %. The predominant respiratory quinone was menaquinone-6 (MK-6). The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major fatty acids of strain DSL-48T were iso-C15 : 0, iso-C17 : 0 3-OH, anteiso-C15 : 0, iso-C15 : 0 3-OH and iso-C16 : 0 3-OH. The G+C content of the genomic DNA was 33.3 mol%. The combined genotypic and phenotypic data indicated that strain DSL-48T represents a novel species of the genus Confluentibacter, for which the name Confluentibacter sediminis sp. nov. is proposed, with the type strain DSL-48T (=KCTC 62648T=MCCC 1K03537T).
ParkS,
KimS,
JungYT,
ParkJM,
YoonJH et al. nov., sp. nov., isolated from the junction between the ocean and a freshwater lake. Int J Syst Evol Microbiol2016; 66:868–873
ParteAC.
LPSN - List of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol2018; 68:1825–1829 [View Article][PubMed]
HanJR,
ZhangH,
ZhengWS,
ChenGJ,
duZJ.
Confluentibacter citreus sp. nov., isolated from lake sediment, and emended description of the genus Confluentibacter
. Int J Syst Evol Microbiol2017; 67:4008–4012 [View Article][PubMed]
GürtlerV,
StanisichVA.
New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology1996; 142:3–16 [View Article][PubMed]
GorisJ,
KonstantinidisKT,
KlappenbachJA,
CoenyeT,
VandammeP et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol2007; 57:81–91 [View Article][PubMed]
WayneLG,
MooreWEC,
StackebrandtE,
KandlerO,
ColwellRR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol1987; 37:463–464 [View Article]
RichterM,
Rosselló-MóraR.
Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA2009; 106:19126–19131 [View Article][PubMed]
WeiY,
FangJ,
XuY,
ZhaoW,
CaoJ.
Corynebacterium hadale sp. nov. isolated from hadopelagic water of the New Britain Trench. Int J Syst Evol Microbiol2018; 68:1474–1478 [View Article][PubMed]
TindallB,
SikorskiJ,
SmibertR,
KriegN.
Phenotypic characterization and the principles of comparative systematics. In
ReddyCA,
BeveridgeTJ,
BreznakJA,
MarzlufGA,
SchmidtTM et al.
(editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society for Microbiology; 2007 pp. 330–393
SkermanV.
A Guide to the Identification of the Genera of Bacteria: with Methods and Digests of Generic Characteristics, 2nd ed. Baltimore, MD: Williams & Wilkins; 1967
MinnikinDE,
O'DonnellAG,
GoodfellowM,
AldersonG,
AthalyeM et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods1984; 2:233–241 [View Article]
Confluentibacter sediminis sp. nov., isolated from the junction between the ocean and a freshwater lake and emended description of the genus Confluentibacter