%0 Journal Article %A Booncharoen, Auttaporn %A Visessanguan, Wonnop %A Kuncharoen, Nattakorn %A Yiamsombut, Supalurk %A Santiyanont, Pannita %A Mhuantong, Wuttichai %A Charoensri, Salisa %A Rojsitthisak, Pornchai %A Tanasupawat, Somboon %T Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi) %D 2019 %J International Journal of Systematic and Evolutionary Microbiology, %V 69 %N 11 %P 3529-3536 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003658 %K moderately halophile %K Ka-pi %K shrimp paste %K Lentibacillus lipolyticus %I Microbiology Society, %X A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9T, was isolated from traditional salted shrimp paste (Ka-pi) produced in Samut Sakhon Province, Thailand. This strain grew optimally at 37–40 °C, pH 7.0 and in the presence of 8–16 % (w/v) NaCl. The 16S rRNA gene sequence similarity values between strain SSKP1-9T and Lentibacillus juripiscarius TISTR 1535T and Lentibacillus halophilus TISTR 1549T were 98.7 and 97.2 %, respectively. Based on 16S rRNA gene sequence similarity, strain SSKP1-9T represents a distinct novel species, as shown by phenotypic traits, DNA–DNA hybridization and average nucleotide identity values. In addition, the whole-cell protein profile confirmed the novelty of the taxon. The genomic DNA G+C content was 44.6 mol%. The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9T represents a novel species of Lentibacillus , for which the name Lentibacilluslipolyticus sp. nov. is proposed. The type strain is SSKP1-9T (=JCM 32625T=TISTR 2597T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003658