1887

Abstract

A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9, was isolated from traditional salted shrimp paste () produced in Samut Sakhon Province, Thailand. This strain grew optimally at 37–40 °C, pH 7.0 and in the presence of 8–16 % (w/v) NaCl. The 16S rRNA gene sequence similarity values between strain SSKP1-9 and TISTR 1535 and TISTR 1549 were 98.7 and 97.2 %, respectively. Based on 16S rRNA gene sequence similarity, strain SSKP1-9 represents a distinct novel species, as shown by phenotypic traits, DNA–DNA hybridization and average nucleotide identity values. In addition, the whole-cell protein profile confirmed the novelty of the taxon. The genomic DNA GC content was 44.6 mol%. The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained -diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were anteiso-C, anteiso-C and iso-C. The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9 represents a novel species of , for which the name sp. nov. is proposed. The type strain is SSKP1-9 (=JCM 32625=TISTR 2597).

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2019-11-01
2019-12-15
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