1887

Abstract

A Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain S1-5-21, was isolated from coastal sediment in Quanzhou Bay, PR China, and subjected to taxonomic characterization. Growth of strain S1-5-21 was observed at 15–40 °C (optimum, 30–35 °C), NaCl tolerance of 0.5–10 % NaCl (w/v; optimum, 1 %), and pH range of 5–8 (optimum, pH 7). Catalase activity and oxidase activity were found to be positive. The nearly complete 16S rRNA gene sequence of strain S1-5-21 shows maximum sequence similarity to MOLA115 (95.7 %), followed by CAU 1040 (90.4 %) and GYP-15 (90.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S1-5-21 forms a distinct monophyletic branch affiliated to the genus The ANI value between strain S1-5-21 and close relative MOLA115 was estimated to be 70.8 %. The respiratory quinone was found to be Q-8. The predominant fatty acids (>10 %) were identified as iso-C, summed feature 9 (iso-C 9 and/or C 10-methyl) and iso-C. The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two aminolipids, three phospholipids and five unidentified polar lipids. The draft genome size of strain S1-5-21 is 5.2 Mb with genomic G+C content of 40.1 mol%. Based on these results, strain S1-5-21 is concluded to represent a novel species within the genus , for which the name sp. nov. is proposed with the type strain S1-5-21 (=MCCC 1K03814=KCTC 72149).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003655
2019-11-01
2020-01-22
Loading full text...

Full text loading...

References

  1. Wang G, Tang M, Wu H, Dai S, Li T et al. Aliikangiella marina gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122, and proposal of Kangiellaceae fam. nov. in the order Oceanospirillales. Int J Syst Evol Microbiol 2015;65:4488–4494 [CrossRef][PubMed]
    [Google Scholar]
  2. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67: [CrossRef][PubMed]
    [Google Scholar]
  3. Lee SY, Park S, Oh TK, Yoon JH. Kangiella sediminilitoris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2013;63:1001–1006 [CrossRef][PubMed]
    [Google Scholar]
  4. Yoon JH, Oh TK, Park YH. Kangiella koreensis gen. nov., sp. nov. and Kangiella aquimarina sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 2004;54:1829–1835 [CrossRef][PubMed]
    [Google Scholar]
  5. Yoon JH, Kang SJ, Lee SY, Lee JS, Oh TK. Kangiella geojedonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012;62:511–514 [CrossRef][PubMed]
    [Google Scholar]
  6. Jean WD, Huang SP, Chen JS, Shieh WY. Kangiella taiwanensis sp. nov. and Kangiella marina sp. nov., marine bacteria isolated from shallow coastal water. Int J Syst Evol Microbiol 2012;62:2229–2234 [CrossRef][PubMed]
    [Google Scholar]
  7. Fagervold SK, Urios L, Intertaglia L, Batailler N, Lebaron P et al. Pleionea mediterranea gen. nov., sp. nov., a gammaproteobacterium isolated from coastal seawater. Int J Syst Evol Microbiol 2013;63:2700–2705 [CrossRef][PubMed]
    [Google Scholar]
  8. Romanenko LA, Tanaka N, Frolova GM, Mikhailov VV. Kangiella japonica sp. nov., isolated from a marine environment. Int J Syst Evol Microbiol 2010;60:2583–2586 [CrossRef][PubMed]
    [Google Scholar]
  9. Xu FD, Li XG, Xiao X, Xu J. Kangiella profundi sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2015;65:2315–2319 [CrossRef][PubMed]
    [Google Scholar]
  10. Kim JH, Ward AC, Kim W. Kangiella chungangensis sp. nov. isolated from a marine sand. Antonie van Leeuwenhoek 2015;107:1291–1298 [CrossRef][PubMed]
    [Google Scholar]
  11. Ahn J, Park JW, Mcconnell JA, Ahn YB, Häggblom MM. Kangiella spongicola sp. nov., a halophilic marine bacterium isolated from the sponge Chondrilla nucula. Int J Syst Evol Microbiol 2011;61:961–964 [CrossRef][PubMed]
    [Google Scholar]
  12. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  13. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19:455–477 [CrossRef][PubMed]
    [Google Scholar]
  14. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015;25:1043–1055 [CrossRef][PubMed]
    [Google Scholar]
  15. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016;66:1100–1103 [CrossRef][PubMed]
    [Google Scholar]
  16. Komagata K, Suzuki K-I. 4 Lipid and cell-wall analysis in bacterial systematics. current methods for classification and identification of microorganisms. Methods in Microbiology 1988;161–207
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003655
Loading
/content/journal/ijsem/10.1099/ijsem.0.003655
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error