1887

Abstract

A Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain S1-5-21, was isolated from coastal sediment in Quanzhou Bay, PR China, and subjected to taxonomic characterization. Growth of strain S1-5-21 was observed at 15–40 °C (optimum, 30–35 °C), NaCl tolerance of 0.5–10 % NaCl (w/v; optimum, 1 %), and pH range of 5–8 (optimum, pH 7). Catalase activity and oxidase activity were found to be positive. The nearly complete 16S rRNA gene sequence of strain S1-5-21 shows maximum sequence similarity to MOLA115 (95.7 %), followed by CAU 1040 (90.4 %) and GYP-15 (90.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S1-5-21 forms a distinct monophyletic branch affiliated to the genus The ANI value between strain S1-5-21 and close relative MOLA115 was estimated to be 70.8 %. The respiratory quinone was found to be Q-8. The predominant fatty acids (>10 %) were identified as iso-C, summed feature 9 (iso-C 9 and/or C 10-methyl) and iso-C. The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two aminolipids, three phospholipids and five unidentified polar lipids. The draft genome size of strain S1-5-21 is 5.2 Mb with genomic G+C content of 40.1 mol%. Based on these results, strain S1-5-21 is concluded to represent a novel species within the genus , for which the name sp. nov. is proposed with the type strain S1-5-21 (=MCCC 1K03814=KCTC 72149).

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2019-11-01
2024-03-28
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