sp. nov., isolated from an ice core Open Access

Abstract

A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated T3246-1, was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China. It grew optimally at 20 °C, pH 7–8 and in the presence of 3 % (w/v) NaCl. The major fatty acid of strain T3246-1 was anteiso-C. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. MK-8 was the dominant isoprenoid quinone. The whole-cell sugars were rhamnose, xylose and mannose. The major cell-wall peptidoglycan was lysine. The genomic DNA G+C content of the strain was 71.4 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1 formed a lineage within the genus and was closely related to YIM 93306 with 95.99 % similarity. The average nucleotide identity value between strain T3246-1 and YIM 93306 was 76.65 %. Based on phenotypic and chemotaxonomic characteristics, strain T3246-1 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T3246-1 (=CGMCC 1.13535=JCM 32923).

Erratum

An erratum has been published for this content:
Corrigendium: sp. nov., isolated from an ice core
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2019-11-01
2024-03-28
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References

  1. Tang SK, Zhi XY, Wang Y, Wu JY, Lee JC et al. Haloactinobacterium album gen. nov., sp. nov., a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov. Int J Syst Evol Microbiol 2010; 60:2113–2119 [View Article][PubMed]
    [Google Scholar]
  2. Yao T, Thompson L, Yang W, Yu W, Gao Y et al. Different glacier status with atmospheric circulations in Tibetan Plateau and surroundings. Nat Clim Chang 2012; 2:663–667 [View Article]
    [Google Scholar]
  3. Zongxiang L, Zhen S, Tandong Y, Wenti W. Resources and distribution of glaciers on the Tibetan Plateau. Resources Sciences 2000; 22:49–52
    [Google Scholar]
  4. Zhang S, Hou S, Yang G, Wang J. Bacterial community in the East Rongbuk Glacier, Mt. Qomolangma (Everest) by culture and culture-independent methods. Microbiol Res 2010; 165:336–345 [View Article][PubMed]
    [Google Scholar]
  5. Reasoner DJ, Geldreich EE. A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 1985; 49:1–7[PubMed]
    [Google Scholar]
  6. Embley TM. The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 1991; 13:171–174 [View Article][PubMed]
    [Google Scholar]
  7. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  8. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
  9. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  10. Smibert RM, Krieg NR. Phenotypic characterization. Methods for General and Molecular Bacteriology 1994607–654
    [Google Scholar]
  11. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
    [Google Scholar]
  12. Hiraishi A, Ueda Y, Ishihara J. Quinone profiling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. Appl Environ Microbiol 1998; 64:992–998[PubMed]
    [Google Scholar]
  13. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477[PubMed]
    [Google Scholar]
  14. Groth I, Schumann P, Weiss N, Martin K, Rainey FA. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996; 46:234–239 [View Article][PubMed]
    [Google Scholar]
  15. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974; 28:226–231[PubMed]
    [Google Scholar]
  16. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article][PubMed]
    [Google Scholar]
  17. Seemann T, Torsten S. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014; 30:2068–2069 [View Article][PubMed]
    [Google Scholar]
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