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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 11

sp. nov., isolated from the faeces of Tibetan antelope () on the Qinghai-Tibet Plateau Free

Abstract

Two novel, Gram-stain-positive, non-motile, facultatively anaerobic, rod-shaped bacteria (strains 2129 and 2119) were isolated from the faeces of Tibetan antelopes () on the Qinghai-Tibet Plateau, PR China. The 16S rRNA gene sequences of the strains showed highest similarity values to DSM 23838 (92.9 and 92.8 %, respectively), and phylogenetic analysis based on 16S rRNA gene and genomic sequences indicated that strains 2129 and 2119 represent a new lineage. Strains 2129 and 2119 could ferment -adonitol and -xylose, but were unable to utilize -mannose and -melibiose nor produce esterase (C4) and proline arylamidase. The G+C contents of the two strains were both 69.0 mol%. Their genomes exhibited less than 40.4 % relatedness in DNA–DNA hybridization tests (below 70 % as the recommended threshold for new species) with all available genomes of the genus in the NCBI database. The major fatty acids of the two strains were Cω9 and C, and the major polar lipids were diphosphatidylglycerol, glycolipid, phosphatidylinositol, phosphatidyl inositol mannoside and phosphoglycolipid. Based on the results of genotypic, phenotypic and biochemical analyses, it is proposed that the two unidentified bacteria be classified as representing a novel species, sp. nov. The type strain is 2129 (=CGMCC 4.7483=DSM 106426).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomyces , novel species , Qinghai-Tibet Plateau and Tibetan antelope
© 2019 The Authors
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2019-11-01
2024-04-25
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Actinomyces lilanjuaniae sp. nov., isolated from the faeces of Tibetan antelope (Pantholops hodgsonii) on the Qinghai-Tibet Plateau
Int J Syst Evol Microbiol 69, 3485 (2019); https://doi.org/10.1099/ijsem.0.003649
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