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Volume 69, Issue 11

sp. nov., isolated from raw cow’s milk Free

Abstract

A Gram-stain-negative bacterial strain, designated CA10, was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain CA10 belonged to the genus , family , and was most closely related to ATCC 27950 (98.75 % similarity). The average nucleotide identity and digital DNA–DNA hybridization values of strain CA10 were 94.4 and 56.9 %, respectively, relative to DSM 16779, being lower than the cut-off values of 95–96 and 70 %, respectively. The predominant respiratory quinone was menaquinone-6; major polar lipid, phosphatidylethanolamine; major fatty acids, iso-C, summed feature 9 (iso-Cω9 and/or C 10-methyl), summed feature 3 (iso-C 2-OH and/or Cω7) and iso-C 3-OH. The results of physiological, chemotaxonomic and biochemical analyses suggested that strain CA10 is a novel species of genus , for which the name sp. nov. is proposed. The type strain is CA10 (=KACC 21234=JCM 33443).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bovine raw milk , Chryseobacterium mulctrae and Chryseobacterium strain CA10T
© 2019 The Authors
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2019-11-01
2024-04-16
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Chryseobacterium mulctrae sp. nov., isolated from raw cow’s milk
Int J Syst Evol Microbiol 69, 3478 (2019); https://doi.org/10.1099/ijsem.0.003647
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