@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003641, author = "Zhang, Xian-Jiao and Feng, Guang-Da and Yao, Qing and Wang, Yong-Hong and Yang, Song-Zhen and Zhu, Hong-Hui", title = "Ramlibacter humi sp. nov., isolated from tropical forest soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "11", pages = "3460-3464", doi = "https://doi.org/10.1099/ijsem.0.003641", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003641", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Ramlibacter", keywords = "draft genome sequence", keywords = "dDDH", keywords = "ANI", keywords = "tropical forest soil", keywords = "Ramlibacter humi", abstract = "A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1T, was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15–37 °C and pH 6.0–8.0 without NaCl. The 16S rRNA gene sequence analyses showed that strain 18x22-1T was closely related to Ramlibacter tataouinensis DSM 14655T (98.5 %), followed by Ramlibacter henchirensis DSM 14656T (97.9 %) and other Ramlibacter species and formed a stable cluster with R. tataouinensis DSM 14655T, R. henchirensis DSM 14656T, Ramlibacter solisilvae JCM 19319T and Ramlibacter rhizophilus CCTCC AB 2015357T. Results of chemotaxonomic analyses showed that ubiquinone-8 (Q-8) was the major respiratory quinone, and the major fatty acids (>10 % of the total amounts) were C16 : 0 and C17 : 0cyclo. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminopholipids and four unidentified phospholipids. The draft genome sequence was 4.47 Mb long with a G+C content of 68.9 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain 18x22-1T and four closely related type strains were in the range of 79.3–82.3 % and 21.9–25.1 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses supported that strain 18x22-1T represents a novel species of the genus Ramlibacter , for which the name Ramlibacter humi sp. nov. is proposed. The type strain is 18x22-1T (=GDMCC 1.1584T=KCTC 52922T).", }