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Volume 69, Issue 11

sp. nov., isolated from tropical forest soil Free

Abstract

A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1, was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15–37 °C and pH 6.0–8.0 without NaCl. The 16S rRNA gene sequence analyses showed that strain 18x22-1 was closely related to DSM 14655 (98.5 %), followed by DSM 14656 (97.9 %) and other species and formed a stable cluster with DSM 14655, DSM 14656, JCM 19319 and s CCTCC AB 2015357. Results of chemotaxonomic analyses showed that ubiquinone-8 (Q-8) was the major respiratory quinone, and the major fatty acids (>10 % of the total amounts) were C and Ccyclo. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminopholipids and four unidentified phospholipids. The draft genome sequence was 4.47 Mb long with a G+C content of 68.9 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain 18x22-1 and four closely related type strains were in the range of 79.3–82.3 % and 21.9–25.1 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses supported that strain 18x22-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 18x22-1 (=GDMCC 1.1584=KCTC 52922).

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Keyword(s): ANI , dDDH , draft genome sequence , Ramlibacter , Ramlibacter humi and tropical forest soil
© 2019 The Authors
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2019-11-01
2024-12-07
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Ramlibacter humi sp. nov., isolated from tropical forest soil
Int J Syst Evol Microbiol 69, 3460 (2019); https://doi.org/10.1099/ijsem.0.003641
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