1887

Abstract

A Gram-stain-negative bacterial strain, designated JW-3, was isolated from a soil sample collected from farmland in Yantai, Shandong Province, PR China. Cells of strain JW-3 are motile rods and strictly aerobic, showing catalase- and oxidase-positive reactions. Strain JW-3 could grow at 16–37 °C (optimum, 30 °C), at pH 6.0–9.0 (pH 7.0) and in the presence of 0–1 % (w/v) NaCl (0.5 %, in Luria–Bertani broth). The major fatty acids were summed feature 3 (C 7 and/or C 6; 35.5 %), iso-C (16.7 %) and C (10.8 %). The major respiratory quinone was ubiquinone-8 (Q8). The polar lipids of strain JW-3 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids, two unidentified lipids, two unidentified glycolipids and a partial unidentified aminophospholipid. Strain JW-3 was most closely related to KA5-B with 97.67 % 16S rRNA gene sequence similarity. Results of phylogenetic analyses, based on 16S rRNA gene sequencing, showed that strain JW-3 forms a distinct phylogenic lineage within the genus of the family . The DNA G+C content of strain JW-3 was 62.57 mol%, based on its draft genome sequence. Average nucleotide identity values and digital DNA–DNA hybridization values for draft genomes, between strain JW-3 and strain KA5-B, were 84.54 and 30.80 %, respectively. Based on its phenotypic, chemotaxonomic and molecular features, and DNA–DNA hybridization results, strain JW-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JW-3 (=CCTCC AB 2018184=KCTC 62820).

Keyword(s): Steroidobacter , new taxa and taxonomy
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/content/journal/ijsem/10.1099/ijsem.0.003639
2019-08-22
2019-10-13
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