sp. nov., a biosurfactant-producing actinobacterium isolated from marine sediment Free

Abstract

A novel actinobacterium producing biosurfactant, designated OTB305, was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305 was closely related to JCM 19630 (98.8 %) and DSM 42084 (98.8 %). The chemotaxonomic characteristics of strain OTB305 corresponded to those of the genus as follows: the diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H) and MK-9(H); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C, C and C 7; and the genomic DNA G+C content was 72.83 mol%. However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305 was distinguished from closely related species. Therefore, strain OTB305 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OTB305 (=NBRC 113255=TBRC 9682).

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2019-12-01
2024-03-28
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References

  1. Waksman SA, Henrici AT. The Nomenclature and Classification of the Actinomycetes. J Bacteriol 1943; 46:337–341[PubMed]
    [Google Scholar]
  2. Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a New Hierarchic Classification System, Actinobacteria classis nov. Int J Syst Bacteriol 1997; 47:479–491 [View Article]
    [Google Scholar]
  3. Kämpfer P. The family Streptomycetaceae, Part I: Taxonomy. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E et al. (editors) The Prokaryotes Vol. 3 New York: Springer; 2006 pp. 538–604 Archaea. Bacteria: Firmicutes, Actinomycetes
    [Google Scholar]
  4. Bérdy J. Bioactive microbial metabolites. J Antibiot 2005; 58:1–26 [View Article][PubMed]
    [Google Scholar]
  5. Morikawa M, Hirata Y, Imanaka T. A study on the structure–function relationship of lipopeptide biosurfactants. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2000; 1488:211–218 [View Article]
    [Google Scholar]
  6. Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM et al. Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods 2004; 56:339–347 [View Article][PubMed]
    [Google Scholar]
  7. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
    [Google Scholar]
  8. Terahara T, Yamada K, Nakayama J, Igarashi Y, Kobayashi T et al. Bacterial community structures of deep-sea water investigated by molecular biological techniques. Gene 2016; 576:696–700 [View Article][PubMed]
    [Google Scholar]
  9. Terahara T, Xu X, Kobayashi T, Imada C. Isolation and characterization of Cr(VI)-reducing actinomycetes from estuarine sediments. Appl Biochem Biotechnol 2015; 175:3297–3309 [View Article][PubMed]
    [Google Scholar]
  10. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  11. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  12. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  13. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  14. Fitch WM. Toward Defining the Course of Evolution: Minimum Change for a Specific Tree Topology. Syst Biol 1971; 20:406–416 [View Article]
    [Google Scholar]
  15. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  16. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
    [Google Scholar]
  17. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983; 29:319–322 [View Article]
    [Google Scholar]
  18. Hamada M, Yamamura H, Komukai C, Tamura T, Suzuki K et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium . J Antibiot 2012; 65:427–431 [View Article][PubMed]
    [Google Scholar]
  19. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the Classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
    [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  21. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
    [Google Scholar]
  22. Pan HQ, Cheng J, Zhang DF, Yu SY, Khieu TN et al. Streptomyces bohaiensis sp. nov., a novel actinomycete isolated from Scomberomorus niphonius in the Bohai Sea. J Antibiot 2015; 68:246–252 [View Article][PubMed]
    [Google Scholar]
  23. Sharma TK, Mawlankar R, Sonalkar VV, Shinde VK, Zhan J et al. Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India. Antonie van Leeuwenhoek 2016; 109:225–235 [View Article][PubMed]
    [Google Scholar]
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