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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 11

sp. nov., isolated from alkaline Lake Dajiaco on the Tibetan Plateau Free

Abstract

A novel Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium was isolated from Lake Dajiaco on the Tibetan Plateau. Strain DJC grew without NaCl and tolerated up to 3 % (w/v) NaCl. Growth occurred at pH 6.0–10.0 (optimum, pH 7.0–8.0) and 15–37 °C (optimum, 25–30 °C). Vitamins were not required for growth. The main polar lipids of strain DJC were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was Q-10. The major fatty acid was Cω7. Genome sequencing revealed a genome size of 4.61 Mbp and a G+C content of 62.9 mol%. Analysis of 16S rRNA sequences showed that strain DJC belonged to the genus , with the closest neighbour RCRI19 (97.5 %). DNA–DNA relatedness between strain DJC and the closest phylogenetically related strain RCRI19 was 40.8 %. Stain DJC was clearly distinguished from the type strain mentioned above through phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristic comparisons. Based on its phenotypic and chemotaxonomic characteristics, strain DJC could be classified as a representative of a novel species of the genus for which the name sp. nov. is proposed. The type strain is DJC (=CICC 24242=KCTC 62173).

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Keyword(s): genotypic and phenotypic analysis , Gram-stain-negative , Lake DajiaCo , Tabrizicola alkalilacus and Tibetan Plateau
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2019-11-01
2024-04-24
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Tabrizicola alkalilacus sp. nov., isolated from alkaline Lake Dajiaco on the Tibetan Plateau
Int J Syst Evol Microbiol 69, 3420 (2019); https://doi.org/10.1099/ijsem.0.003635
/content/journal/ijsem/10.1099/ijsem.0.003635
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