1887

Abstract

A Gram-stain-negative, non-motile bacterial strain, designated SW125, was isolated from a deep-sea vsediment sample collected from the Indian Ocean. Cells were aerobic, catalase-positive and oxidase-positive. The strain grew at 4–42 °C (optimum, 30 °C), at pH 5.0–10.0 (optimum, pH 7.0–7.6) and in the presence of 0.5–9.0 % (w/v) NaCl (optimum, 2.0–3.0 %). Comparative analyses of the 16S rRNA gene sequence showed that strain SW125 was grouped in the vicinity of the genus with the highest sequence similarity of 91.6 % to the type strain of . The quinone system contained menaquinone MK-7 as the predominant component. The predominant cellular fatty acids of strain SW125 were iso-C, iso-C 3-OH and summed feature 3 (comprising Cω7 and/or Cω6). Strain SW125 contained phosphatidylethanolamine as the major polar lipid. The genomic DNA G+C content of strain SW125 was 43.2 mol%. On the basis of its phenotypic characteristics and phylogenetic data, strain SW125 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is SW125 (=CGMCC 1.16172=DSM 107180=KCTC 62636).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003633
2019-11-05
2019-11-13
Loading full text...

Full text loading...

References

  1. Ludwig W, Euzéby J, Whitman WB. Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes. In Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 4 New York: Springer; 2010; vol.
    [Google Scholar]
  2. Kämpfer P, Young CC, Chen WM, Rekha PD, Fallschissel K et al. Fontibacter flavus gen. nov., sp. nov., a member of the family 'Cyclobacteriaceae', isolated from a hot spring. Int J Syst Evol Microbiol 2010;60: 2066– 2070 [CrossRef] [PubMed]
    [Google Scholar]
  3. Bhumika V, Srinivas TN, Ravinder K, Kumar PA. Mariniradius saccharolyticus gen. nov., sp. nov., a member of the family Cyclobacteriaceae isolated from marine aquaculture pond water, and emended descriptions of the genus Aquiflexum and Aquiflexum balticum. Int J Syst Evol Microbiol 2013;63: 2088– 2094 [CrossRef] [PubMed]
    [Google Scholar]
  4. Wang YX, Liu YP, Huo QQ, Li YP, Feng FY et al. Mongoliibacter ruber gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae isolated from a haloalkaline lake. Int J Syst Evol Microbiol 2016;66: 1088– 1094 [CrossRef] [PubMed]
    [Google Scholar]
  5. Han JR, Geng QL, Wang FQ, du ZJ, Chen GJ. Algoriphagus marinus sp. nov., isolated from marine sediment and emended description of the genus Algoriphagus. Int J Syst Evol Microbiol 2017;67: 2412– 2417 [CrossRef] [PubMed]
    [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  7. Song L, Liu H, Cai S, Zhou Y. Marinimicrobium alkaliphilum sp. nov., an alkaliphilic bacterium isolated from soil and emended description of the genus Marinimicrobium. Int J Syst Evol Microbiol 2019;69: 481– 485 [CrossRef] [PubMed]
    [Google Scholar]
  8. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25: 4876– 4882 [CrossRef] [PubMed]
    [Google Scholar]
  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  10. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4: 406– 425 [CrossRef] [PubMed]
    [Google Scholar]
  11. Fitch WM. Toward defining the course of evolution: minimum change for a Specific Tree Topology. Syst Zool197: 406– 416
    [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  13. Dong X, Cai M. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  14. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982;16: 584– 586 [PubMed]
    [Google Scholar]
  15. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988;38: 358– 361 [CrossRef]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  17. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66: 199– 202 [CrossRef]
    [Google Scholar]
  18. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, A. G. O’Donnell. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994; pp. 121– 161
    [Google Scholar]
  19. Hu B, Yang Q, Cai M, Tang YQ, Zhao GF et al. Negadavirga shengliensis gen. nov., sp. nov., a novel member of the family Cyclobacteriaceae isolated from oil-contaminated saline soil. Antonie Van Leeuwenhoek 2015;107: 663– 673 [CrossRef] [PubMed]
    [Google Scholar]
  20. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015;25: 1043– 1055 [CrossRef] [PubMed]
    [Google Scholar]
  21. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68: 461– 466 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003633
Loading
/content/journal/ijsem/10.1099/ijsem.0.003633
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error