1887

Abstract

A white-coloured, Gram-stain-negative, aerobic, rod-shaped bacterium (designated strain SY21) was isolated from waste-activated sludge. Optimal growth occurred at 28 °C and pH 7.0. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain SY21 exhibited 16S rRNA gene sequence similarities of 95.5–98.0 % to species and clustered with the type species of the genus . In strain SY21, the predominant respiratory quinone was ubiquinone Q-8, and the cellular fatty acids consisted mainly of iso-C, C, iso-C 3-OH, summed feature 3 and summed feature 9. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was determined to be 67.9 mol% and the DNA–DNA relatedness between strain SY21 and the closest phylogenetically related strain, KCTC 42013, was 35.0±0.1 %. Based on the distinct phenotypic, chemotaxonomic and phylogenetic properties, strain SY21 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SY21 (=KCTC 62191=NBRC 113114).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003630
2019-11-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/11/3399.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003630&mimeType=html&fmt=ahah

References

  1. Busse HJ, Kämpfer P, Moore ER, Nuutinen J, Tsitko IV et al. Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry. Int J Syst Evol Microbiol 2002; 52:473–483 [View Article][PubMed]
    [Google Scholar]
  2. Mergaert J, Cnockaert MC, Swings J. Thermomonas fusca sp. nov. and Thermomonas brevis sp. nov., two mesophilic species isolated from a denitrification reactor with poly(epsilon-caprolactone) plastic granules as fixed bed, and emended description of the genus Thermomonas . Int J Syst Evol Microbiol 2003; 53:1961–1966 [View Article][PubMed]
    [Google Scholar]
  3. Alves MP, Rainey FA, Nobre MF, da Costa MS. Thermomonas hydrothermalis sp. nov., a new slightly thermophilic gamma-proteobacterium isolated from a hot spring in central Portugal. Syst Appl Microbiol 2003; 26:70–75 [View Article][PubMed]
    [Google Scholar]
  4. Kim MK, Im WT, In JG, Kim SH, Yang DC. Thermomonas koreensis sp. nov., a mesophilic bacterium isolated from a ginseng field. Int J Syst Evol Microbiol 2006; 56:1615–1619 [View Article][PubMed]
    [Google Scholar]
  5. Wang L, Zheng S, Wang D, Wang L, Wang G. Thermomonas carbonis sp. nov., isolated from the soil of a coal mine. Int J Syst Evol Microbiol 2014; 64:3631–3635 [View Article][PubMed]
    [Google Scholar]
  6. Wagner M, Loy A. Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol 2002; 13:218–227 [View Article][PubMed]
    [Google Scholar]
  7. Xu S, Yao J, Ainiwaer M, Hong Y, Zhang Y. Analysis of bacterial community structure of activated sludge from wastewater treatment plants in winter. Biomed Res Int 2018; 2018:1–8 [View Article][PubMed]
    [Google Scholar]
  8. Chun J, Goodfellow M. A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 1995; 45:240–245 [View Article][PubMed]
    [Google Scholar]
  9. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial systematics Chichester: Wiley; 1991 pp. 115–175
    [Google Scholar]
  10. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  11. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  12. Kimura M. The Neutral Theory of Molecular Evolution New York: Cambridge University Press; 1983
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  14. Cavalli-Sforza LL, Edwards AW. Phylogenetic analysis. Models and estimation procedures. Am J Hum Genet 1967; 19:233–257[PubMed]
    [Google Scholar]
  15. Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  16. Felsenstein J. Phylogenies and the comparative method. Am Nat 1985; 125:1–15 [View Article]
    [Google Scholar]
  17. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  18. Stackebrandt E, Goebel BM. Taxonomic Note: A Place for DNA-DNA reassociation and 16s rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  19. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
    [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  21. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207
    [Google Scholar]
  22. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  23. Yassin AF, Chen WM, Hupfer H, Siering C, Kroppenstedt RM et al. Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 2007; 57:1131–1136 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.003630
Loading
/content/journal/ijsem/10.1099/ijsem.0.003630
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error