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Abstract

Strains A31/70, CCUG 58779 and SD129 were Gram-stain-negative, short rods, motile by one polar flagellum and isolated from clinical specimens in Botswana, Sweden and Spain, respectively. The 16S rRNA sequence similarity values grouped them in the phylogenetic group of species. The DNA G+C content ranged from 65.5 to 65.7 mol%. The strains were characterized phenotypically by the API 20NE and Biolog GEN III tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and by their fatty acid contents. The absence of the arginine dihydrolase and the hydrolysis of gelatin differentiated these strains from the closest species, . The major fatty acid contents were summed feature 8 (38.6 %), C (22.6 %), summed feature 3 (20.5 %) and C (8.4 %). Multilocus sequence analysis with three housekeeping gene sequences (, and 16S rRNA) together with whole-genome comparisons indicated that these strains cluster together in the phylogenetic analysis and their similarity values were lower than the thresholds established for species in the genus . These results permit us to conclude that strains A31/70, CCUG 58779 and SD129 belong to a novel species in the genus for which the name sp. nov. is proposed. The type strain is A31/70 (=CECT 9981=CCUG 73638).

Keyword(s): Pseudomonas and nosocomial
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/content/journal/ijsem/10.1099/ijsem.0.003628
2019-08-02
2019-09-22
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