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Abstract

A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated ZQ420, was isolated from marine sediment sampled on Zhoushan Island located in the East China Sea. Strain ZQ420 was able to grow at 10–45 °C, 0–12.0 % (w/v) NaCl and pH 5.5–9.0. Catalase and oxidase activities, nitrate reduction, HS production, hydrolysis of starch, casein, Tween 20, 40 and 80 were positive. Indole, methyl red, Voges–Proskauer test, hydrolysis of gelatin and Tween 60 were negative. The major cellular fatty acids were C 7, C and 11-methyl Cω7. Ubiquinone-10 (Q-10) was the only detected respiratory quinone. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids, two unidentified aminolipid and two unidentified lipids. The DNA G+C content was 64.8 mol%. According to 16S rRNA gene sequence similarities, strain ZQ420 shared 97.9, 96.3 and 96.3 % similarities to the following species with validated names D1-19, PAMC27389 and 4-T-34, respectively. While sharing lower sequence similarities (<96.0 %) to other type species. Phylogenetic analyses showed that strain ZQ420 and D1-19 formed an independent cluster in the phylogenetic trees. The average nucleotide identity value between strain ZQ420 and D1-19 was 79.1 %. The DNA–DNA hybridization analysis revealed that strain ZQ420 shared 21.5 % DNA relatedness with D1-19. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain ZQ420 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is ZQ420 (=KCTC 62579=MCCC 1K03530).

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/content/journal/ijsem/10.1099/ijsem.0.003627
2019-10-01
2019-10-15
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