sp. nov., isolated from a rhizosphere mudflat of a halophyte () Free

Abstract

The taxonomic status of a Gram-reaction-negative, aerobic, motile, rod-shaped bacterium, designated strain GH3-15, was examined by a polyphasic approach. The strain, which was isolated from the rhizosphere mudflat of a halophyte at the seashore of Gangwha Island, Republic of Korea, was found to belong to the family based on 16S rRNA gene sequences. The closest phylogenetic neighbour was SM1501 (98.3 % sequence similarity). Levels of 16S rRNA gene sequence similarity of strain GH3-15 to other members of the family were <97.1 %. The respiratory quinone was Q-10. The major fatty acids were Cω6, Cω7, C 2-OH, 11-methyl Cω7 and C. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The novel isolate exhibited growth at 20–40 °C, at pH 5–9, and in the presence of 1–7 % (w/v) NaCl. DNA relatedness between strain GH3-15 and its closet relative was 32.9±8.8 %. On the basis of phenotypic, chemotaxonomic and DNA–DNA hybridization data, in addition to a distinct phylogenetic position, strain GH3-15 (=KCTC 62380=JCM 32445) represents a novel species of the genus for which the name sp. nov. is proposed.

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2019-10-01
2024-03-28
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